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BMRB: 25448

2MY9

Solution structure of N-terminal domain of human TIG3

Authors

Wei, H., Wang, L., Xia, B.

Assembly

N-terminal domain of human TIG3

Entity

1. N-terminal domain of human TIG3 (polymer, Thiol state: all free), 125 monomers, 14032.73 Da Detail

MASPHQEPKP GDLIEIFRLG YEHWALYIGD GYVIHLAPPS EYPGAGSSSV FSVLSNSAEV KRERLEDVVG GCCYRVNNSL DHEYQPRPVE VIISSAKEMV GQKMKYSIVS RNCEHFVTQL RYGKS

Formula weight

14032.73 Da

Source organism

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 77.7 %, Completeness (bb): 78.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.7 % (1124 of 1446)	83.7 % (631 of 754)	67.7 % (383 of 566)	87.3 % (110 of 126)
Backbone	78.5 % (576 of 734)	90.9 % (229 of 252)	67.4 % (246 of 365)	86.3 % (101 of 117)
Sidechain	79.4 % (657 of 827)	80.1 % (402 of 502)	77.8 % (246 of 316)	100.0 % (9 of 9)
Aromatic	27.8 % (35 of 126)	44.4 % (28 of 63)	9.7 % (6 of 62)	100.0 % (1 of 1)
Methyl	95.9 % (117 of 122)	96.7 % (59 of 61)	95.1 % (58 of 61)

1. TIG3N

MASPHQEPKP GDLIEIFRLG YEHWALYIGD GYVIHLAPPS EYPGAGSSSV FSVLSNSAEV KRERLEDVVG GCCYRVNNSL DHEYQPRPVE VIISSAKEMV GQKMKYSIVS RNCEHFVTQL RYGKS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MY9, Strand ID: A Detail

Release date

2015-04-06

Citation

Structural and functional characterization of tumor suppressors TIG3 and H-REV107
Ren, X., Wei, H., Wang, L., Yu, W., Lin, J., Jin, C., Xia, B.
FEBS Lett. (2015), 589, 1179-1186, PubMed 25871522 , DOI 10.1016/j.febslet.2015.04.002 , Abstract

Related entities 1. N-terminal domain of human TIG3, : 1 : 3 : 1 : 65 entities Detail

Interaction partners 1. N-terminal domain of human TIG3, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

5 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

9 3D HCCH-COSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TIG3N	[U-13C; U-15N]		0.5 mM
2	DSS	natural abundance		0.03 % w/v
3	DTT	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	potassium chloride	natural abundance		50 mM
6	D2O	natural abundance		10 %
7	H2O	natural abundance		90 %

nullKeywords H-REV107 family, NlpC/P60, TIG3, phospholipase