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Found 1 Document (0.926 seconds)

BMRB: 25469

2MZ1

Solution structure of hnRNP C RRM in complex with 5'-UUUUC-3' RNA

Authors

Cienikova, Z., Damberger, F.F., Hall, J., Allain, F.H-T., Maris, C.

Assembly

hnRNP C RRM in complex with 5'-UUUUC-3' RNA

Entity

1. hnRNP C RRM in complex with 5'-UUUUC-3' RNA, entity 1 (polymer, Thiol state: all free), 105 monomers, 11274.78 Da Detail

ASNVTNKTDP RSMNSRVFIG NLNTLVVKKS DVEAIFSKYG KIVGCSVHKG FAFVQYVNER NARAAVAGED GRMIAGQVLD INLAAEPKVN RGKAGVKRSA AEMYG

2. RNA (5'-R(*UP*UP*UP*UP*C)-3') (polymer, Thiol state: not present), 5 monomers, 1547.860 Da Detail

UUUUC

Total weight

12822.641 Da

Max. entity weight

11274.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.4 %, Completeness (bb): 95.9 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	94.4 % (1192 of 1263)	99.1 % (651 of 657)	87.7 % (428 of 488)	95.8 % (113 of 118)
Backbone	95.9 % (653 of 681)	99.6 % (247 of 248)	92.1 % (304 of 330)	99.0 % (102 of 103)
Sidechain	93.6 % (634 of 677)	98.8 % (404 of 409)	86.6 % (219 of 253)	73.3 % (11 of 15)
Aromatic	59.4 % (57 of 96)	93.8 % (45 of 48)	27.3 % (12 of 44)	0.0 % (0 of 4)
Methyl	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (60 of 60)

1. entity 1

ASNVTNKTDP RSMNSRVFIG NLNTLVVKKS DVEAIFSKYG KIVGCSVHKG FAFVQYVNER NARAAVAGED GRMIAGQVLD INLAAEPKVN RGKAGVKRSA AEMYG

2. RNA (5'-R(*UP*UP*UP*UP*C)-3')

UUUUC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 15N]	1.0 mM
2	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
6	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	10 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MZ1, Strand ID: A Detail

Release date

2015-04-08

Citation

Structural and mechanistic insights into poly(uridine) tract recognition by the hnRNP C RNA recognition motif
Cienikova, Z., Damberger, F.F., Hall, J., Allain, F.H-T., Maris, C.
J. Am. Chem. Soc. (2014), 136, 14536-14544, PubMed 25216038 , DOI 10.1021/ja507690d , Abstract

Related entities 1. hnRNP C RRM in complex with 5'-UUUUC-3' RNA, entity 1, : 1 : 8 : 60 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 15N]	1.0 mM
2	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

3 3D HNCACO

Instrument

Bruker AVIII - 700 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
6	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	10 mM

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 15N]	1.0 mM
2	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM

5 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

6 3D 1H-13C edited-filtered NOESY

Instrument

Bruker AVIII - 700 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

7 2D 1H-1H TOCSY

Instrument

Bruker AVIII - 700 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

8 2D 1H-1H NOESY filtered-edited

Instrument

Bruker AVIII - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

9 2D 1H-1H NOESY filtered-filtered

Instrument

Bruker AVIII - 500 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	entity_1	[U-100% 13C; U-100% 15N]	1.0 mM
10	RNA (5'-R(UPUPUPUP*C)-3')	natural abundance	1.0 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	10 mM

nullKeywords RNA, RRM, complex, hnRNP C

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Found 1 Document (0.926 seconds)

BMRB: 25469

Sample #1

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Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. hnRNP C RRM in complex with 5'-UUUUC-3' RNA, entity 1, : 1 : 8 : 60 entities Detail

Experiments performed 9 experiments Detail

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