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BMRB: 25476

2MZ8

Solution NMR structure of Salmonella typhimurium transcriptional regulator protein Crl

Authors

Cavaliere, P., Levi-Acobas, F., Monteil, V., Bellalou, J., Mayer, C., Norel, F., Sizun, C.

Assembly

His ST-Crl

Entity

1. His ST-Crl (polymer, Thiol state: all free), 154 monomers, 18142.20 Da Detail

HHHHHHSSGT GSGENLYFQG HMTLPSGHPK SRLIKKFTAL GPYIREGQCE DNRFFFDCLA VCVNVKPAPE KREFWGWWME LEAQEKRFTY RYQFGLFDKE GNWTVVPINE TEVVERLEYT LREFHEKLRD LLISMELALE PSDDFNDEPV KLSA

Formula weight

18142.2 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 81.1 %, Completeness (bb): 88.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.1 % (1518 of 1872)	81.5 % (797 of 978)	79.4 % (583 of 734)	86.3 % (138 of 160)
Backbone	88.8 % (806 of 908)	89.4 % (277 of 310)	88.7 % (401 of 452)	87.7 % (128 of 146)
Sidechain	76.0 % (842 of 1108)	77.8 % (520 of 668)	73.2 % (312 of 426)	71.4 % (10 of 14)
Aromatic	52.1 % (122 of 234)	71.8 % (84 of 117)	30.1 % (34 of 113)	100.0 % (4 of 4)
Methyl	97.8 % (131 of 134)	97.0 % (65 of 67)	98.5 % (66 of 67)

1. His ST-Crl

HHHHHHSSGT GSGENLYFQG HMTLPSGHPK SRLIKKFTAL GPYIREGQCE DNRFFFDCLA VCVNVKPAPE KREFWGWWME LEAQEKRFTY RYQFGLFDKE GNWTVVPINE TEVVERLEYT LREFHEKLRD LLISMELALE PSDDFNDEPV KLSA

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
1	His_ST-Crl	[U-98% 15N]	0.3 (±0.03) mM
2	Sodium phosphate	natural abundance	50.0 (±5.0) mM
3	Sodium chloride	natural abundance	300.0 (±30.0) mM
4	Dithiotreitol	natural abundance	2.0 (±0.2) mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

Sample #3

Solvent system 100% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
13	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.35 (±0.04) mM
14	Sodium phosphate	natural abundance	50.0 (±5.0) mM
15	Sodium chloride	natural abundance	300.0 (±30.0) mM
16	Dithiotreitol	natural abundance	2.0 (±0.2) mM
17	D2O	natural abundance	100 %

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
18	His_ST-Crl	[U-100% 13C; U-100% 15N; U-80% 2H]	0.300 (±0.03) mM
19	Sodium phosphate	natural abundance	50.000 (±5.0) mM
20	Sodium chloride	natural abundance	300.000 (±30.0) mM
21	Dithiotreitol	natural abundance	2.0 (±0.2) mM
22	D2O	natural abundance	7 %
23	H2O	natural abundance	93 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291

Referencing offset: 0.35 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MZ8, Strand ID: A Detail

Release date

2015-12-20

Citation

Binding interface between the Salmonella σ(S)/RpoS subunit of RNA polymerase and Crl: hints from bacterial species lacking crl
Cavaliere, P., Sizun, C., Levi-Acobas, F., Nowakowski, M., Monteil, V., Bontems, F., Bellalou, J., Mayer, C., Norel, F.
Sci. Rep. (2015), 5, 13564-13564, PubMed 26338235 , DOI 10.1038/srep13564 , Abstract

Related entities 1. His ST-Crl, : 1 : 5 : 21 entities Detail

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
1	His_ST-Crl	[U-98% 15N]	0.3 (±0.03) mM
2	Sodium phosphate	natural abundance	50.0 (±5.0) mM
3	Sodium chloride	natural abundance	300.0 (±30.0) mM
4	Dithiotreitol	natural abundance	2.0 (±0.2) mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
1	His_ST-Crl	[U-98% 15N]	0.3 (±0.03) mM
2	Sodium phosphate	natural abundance	50.0 (±5.0) mM
3	Sodium chloride	natural abundance	300.0 (±30.0) mM
4	Dithiotreitol	natural abundance	2.0 (±0.2) mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

3 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	His_ST-Crl	[U-98% 15N]	0.3 (±0.03) mM
2	Sodium phosphate	natural abundance	50.0 (±5.0) mM
3	Sodium chloride	natural abundance	300.0 (±30.0) mM
4	Dithiotreitol	natural abundance	2.0 (±0.2) mM
5	D2O	natural abundance	7 %
6	H2O	natural abundance	93 %

4 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 6.500

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

5 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 6.500

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

6 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

7 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

8 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

9 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

10 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
7	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.25 (±0.25) mM
8	Sodium phosphate	natural abundance	50.0 (±5.0) mM
9	Sodium chloride	natural abundance	300.0 (±30.0) mM
10	Dithiotreitol	natural abundance	2.0 (±0.2) mM
11	D2O	natural abundance	7 %
12	H2O	natural abundance	93 %

11 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
18	His_ST-Crl	[U-100% 13C; U-100% 15N; U-80% 2H]	0.300 (±0.03) mM
19	Sodium phosphate	natural abundance	50.000 (±5.0) mM
20	Sodium chloride	natural abundance	300.000 (±30.0) mM
21	Dithiotreitol	natural abundance	2.0 (±0.2) mM
22	D2O	natural abundance	7 %
23	H2O	natural abundance	93 %

12 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
18	His_ST-Crl	[U-100% 13C; U-100% 15N; U-80% 2H]	0.300 (±0.03) mM
19	Sodium phosphate	natural abundance	50.000 (±5.0) mM
20	Sodium chloride	natural abundance	300.000 (±30.0) mM
21	Dithiotreitol	natural abundance	2.0 (±0.2) mM
22	D2O	natural abundance	7 %
23	H2O	natural abundance	93 %

13 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
18	His_ST-Crl	[U-100% 13C; U-100% 15N; U-80% 2H]	0.300 (±0.03) mM
19	Sodium phosphate	natural abundance	50.000 (±5.0) mM
20	Sodium chloride	natural abundance	300.000 (±30.0) mM
21	Dithiotreitol	natural abundance	2.0 (±0.2) mM
22	D2O	natural abundance	7 %
23	H2O	natural abundance	93 %

14 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
18	His_ST-Crl	[U-100% 13C; U-100% 15N; U-80% 2H]	0.300 (±0.03) mM
19	Sodium phosphate	natural abundance	50.000 (±5.0) mM
20	Sodium chloride	natural abundance	300.000 (±30.0) mM
21	Dithiotreitol	natural abundance	2.0 (±0.2) mM
22	D2O	natural abundance	7 %
23	H2O	natural abundance	93 %

15 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
18	His_ST-Crl	[U-100% 13C; U-100% 15N; U-80% 2H]	0.300 (±0.03) mM
19	Sodium phosphate	natural abundance	50.000 (±5.0) mM
20	Sodium chloride	natural abundance	300.000 (±30.0) mM
21	Dithiotreitol	natural abundance	2.0 (±0.2) mM
22	D2O	natural abundance	7 %
23	H2O	natural abundance	93 %

16 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
13	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.35 (±0.04) mM
14	Sodium phosphate	natural abundance	50.0 (±5.0) mM
15	Sodium chloride	natural abundance	300.0 (±30.0) mM
16	Dithiotreitol	natural abundance	2.0 (±0.2) mM
17	D2O	natural abundance	100 %

17 3D hCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
13	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.35 (±0.04) mM
14	Sodium phosphate	natural abundance	50.0 (±5.0) mM
15	Sodium chloride	natural abundance	300.0 (±30.0) mM
16	Dithiotreitol	natural abundance	2.0 (±0.2) mM
17	D2O	natural abundance	100 %

18 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
13	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.35 (±0.04) mM
14	Sodium phosphate	natural abundance	50.0 (±5.0) mM
15	Sodium chloride	natural abundance	300.0 (±30.0) mM
16	Dithiotreitol	natural abundance	2.0 (±0.2) mM
17	D2O	natural abundance	100 %

19 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 8.000

#	Name	Isotope labeling	Concentration
13	His_ST-Crl	[U-98% 13C; U-98% 15N]	0.35 (±0.04) mM
14	Sodium phosphate	natural abundance	50.0 (±5.0) mM
15	Sodium chloride	natural abundance	300.0 (±30.0) mM
16	Dithiotreitol	natural abundance	2.0 (±0.2) mM
17	D2O	natural abundance	100 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25476_2mz8.nef
Input source #2: Coordindates	2mz8.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-----------------------------10--------20--------30--------40--------50--------60--------70--------8
HHHHHHSSGTGSGENLYFQGHMTLPSGHPKSRLIKKFTALGPYIREGQCEDNRFFFDCLAVCVNVKPAPEKREFWGWWMELEAQEKRFTYRYQFGLFDKE
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HHHHHHSSGTGSGENLYFQGHMTLPSGHPKSRLIKKFTALGPYIREGQCEDNRFFFDCLAVCVNVKPAPEKREFWGWWMELEAQEKRFTYRYQFGLFDKE
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

0--------90-------100-------110-------120-------130---
GNWTVVPINETEVVERLEYTLREFHEKLRDLLISMELALEPSDDFNDEPVKLSA
||||||||||||||||||||||||||||||||||||||||||||||||||||||
GNWTVVPINETEVVERLEYTLREFHEKLRDLLISMELALEPSDDFNDEPVKLSA
-------110-------120-------130-------140-------150----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	154	0	0	100.0

Content subtype: combined_25476_2mz8.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list	Warning	1488		96.1 (chain: A, length: 154)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	2371 (1)	noe	79.9 (chain: A, length: 154)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_3	OK	217 (217)	.	81.2 (chain: A, length: 154)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 154, Sequence coverage: 96.1%
- Total number of assignments
  - ¹H chemical shifts: 787
  - ¹³C chemical shifts: 570
  - ¹⁵N chemical shifts: 131
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
27	GLN	CD	179.15

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	978	787	80.5
¹³C chemical shifts	734	569	77.5
¹⁵N chemical shifts	169	131	77.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	310	274	88.4
¹³C chemical shifts	308	265	86.0
¹⁵N chemical shifts	146	121	82.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	668	513	76.8
¹³C chemical shifts	426	304	71.4
¹⁵N chemical shifts	23	10	43.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	69	98.6
¹³C chemical shifts	70	69	98.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	117	82	70.1
¹³C chemical shifts	113	32	28.3
¹⁵N chemical shifts	4	4	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 154, Sequence coverage: 79.9%
- Total number of restraints: 2369
- Weight of restraints: 1.0 (2369)
- Potential type of restraints: upper-bound-parabolic (2369)
- Range of distance target values: 2.28, 6.52 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 154, Sequence coverage: 81.2%
- Total number of restraints: 217
- Total number of restraints per polymer type: 217
- Weight of restraints: 1.0 (217)
- Potential type of restraints: square-well-parabolic (217)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Crl, Salmonella enterica, curli, serovar typhimurium, sigma factor activator, sigma factor binding protein, sigmaS, stationary phase, stress response, transcriptional regulator, typhimurium, sigmaS chaperone, sigma factor activation, RNA polymerase

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Found 1 Document (0.808 seconds)

BMRB: 25476

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. His ST-Crl, : 1 : 5 : 21 entities Detail

Experiments performed 19 experiments Detail

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NMR combined restraints 4 contents Detail