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BMRB: 25492

2MZN

NMR structure of the HLTF HIRAN domain in its DNA-bound conformation.

Authors

Korzhnev, D., Eldirany, S.

Assembly

HIRAN

Entity

1. HIRAN (polymer, Thiol state: not present), 122 monomers, 13514.17 Da Detail

GSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KL

Formula weight

13514.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 84.7 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.7 % (1203 of 1420)	83.5 % (617 of 739)	86.4 % (470 of 544)	84.7 % (116 of 137)
Backbone	96.4 % (698 of 724)	97.2 % (245 of 252)	95.2 % (337 of 354)	98.3 % (116 of 118)
Sidechain	75.3 % (607 of 806)	76.4 % (372 of 487)	78.3 % (235 of 300)	0.0 % (0 of 19)
Aromatic	3.6 % (4 of 110)	3.6 % (2 of 55)	3.7 % (2 of 54)	0.0 % (0 of 1)
Methyl	98.5 % (130 of 132)	98.5 % (65 of 66)	98.5 % (65 of 66)

1. entity

GSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	57.0 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	119.0 ppm	internal	indirect	1.0

Referencing offset: -1.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	57.0 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	119.0 ppm	internal	indirect	1.0

Referencing offset: -1.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	57.0 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	119.0 ppm	internal	indirect	1.0

Referencing offset: 0.31 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	57.0 ppm	internal	indirect	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	119.0 ppm	internal	indirect	1.0

Referencing offset: -0.71 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MZN, Strand ID: A Detail

Release date

2015-06-28

Citation

HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal
Kile, A., Chavez, D.A., Bacal, J., Korzhnev, D., Eldirany, S., Bezsonova, I., Eichman, B.F., Cimprich, K.A.
Mol. Cell (2015), 58, 1090-1100, PubMed 26051180 , DOI 10.1016/j.molcel.2015.05.013 , Abstract

Related entities 1. HIRAN, : 1 : 1 : 6 : 11 entities Detail

Interaction partners 1. HIRAN, : 30 interactors Detail

More details for 30 interactors identified by 14 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC