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BMRB: 25504

2MZW

Staphylococcus aureus FusB:EF-GC3 complex.

Authors

Tomlinson, J.H., Thompson, G.S., Kalverda, A.P., Zhuravleva, A., O'Neill, A.

Assembly

Staphylococcus aureus FusB:EF-GC3 complex

Entity

1. EF-GC3 (polymer, Thiol state: all free), 292 monomers, 32234.15 Da Detail

MEFPEPVIHL SVEPKSKADQ DKMTQALVKL QEEDPTFHAH TDEETGQVII GGMGELHLDI LVDRMKKEFN VECNVGAPMV SYRETFKSSA QVQGKFSRQS GGRGQYGDVH IEFTPNETGA GFEFENAIVG GVVPREYIPS VEAGLKDAME NGVLAGYPLI DVKAKLYDGS YHDVDSSEMA FKIAASLALK EAAKKCDPVI LEPMMKVTIE MPEEYMGDIM GDVTSRRGRV DGMEPRGNAQ VVNAYVPLSE MFGYATSLRS NTQGRGTYTM YFDHYAEVPK SIAEDIIKKN KG

2. FusB (polymer, Thiol state: all other bound), 233 monomers, 27309.00 Da Detail

MGSSHHHHHH SSGLVPNGSH MKTMIYPHQY NYIRSVILRL KNVYKTVNDK ETVKVIQSET YNDINEIFGH IDDDIEESLK VLMNIRLSNK EIEAILNKFL EYVVPFELPS PQKLQKVFKK VKKIKIPQFE EYDLKVSSFV GWNELASNRK YIIYYDEKKQ LKGLYGEISN QVVKGFCTIC NKESNVSLFM KKSKTNSDGQ YVKKGDYICR DSIHCNKQLT DINQFYNFID KLD

3. Staphylococcus aureus FusB:EF-GC3 complex, entity ZN (non-polymer), 65.409 Da

Total weight

59608.56 Da

Max. entity weight

32234.15 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:CYS177:SG	3:ZN1:ZN
2	na	sing	2:CYS180:SG	3:ZN1:ZN
3	na	sing	2:CYS209:SG	3:ZN1:ZN
4	na	sing	2:CYS215:SG	3:ZN1:ZN

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 62.7 %, Completeness: 18.0 %, Completeness (bb): 30.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	18.0 % (1119 of 6221)	14.5 % (472 of 3251)	14.2 % (344 of 2418)	54.9 % (303 of 552)
Backbone	30.6 % (950 of 3108)	32.2 % (343 of 1066)	19.9 % (306 of 1538)	59.7 % (301 of 504)
Sidechain	5.5 % (197 of 3601)	5.9 % (129 of 2185)	4.8 % (66 of 1368)	4.2 % (2 of 48)
Aromatic	2.8 % (14 of 498)	4.0 % (10 of 249)	1.6 % (4 of 248)	0.0 % (0 of 1)
Methyl	5.3 % (28 of 528)	6.1 % (16 of 264)	4.5 % (12 of 264)

1. EF-GC3

2. FusB

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
1	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
2	FusB	natural abundance	0.375 (±0.01) mM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
6	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
7	FusB	natural abundance	0.375 (±0.01) mM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	300 mM
10	DTT	natural abundance	5 mM
11	Pf1 phage	natural abundance	6 mg/mL

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details FusB R19C mutant tagged with MTSL; 20 mM TrisHCl, 300 mM NaCl, pH 8.0

#	Name	Isotope labeling	Concentration
12	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
13	FusB	natural abundance	0.375 (±0.01) mM
14	TRIS	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	DTT	natural abundance	5 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details FusB T26C mutant tagged with MTSL; 20 mM TrisHCl, 300 mM NaCl, pH 8.0

#	Name	Isotope labeling	Concentration
17	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
18	FusB	natural abundance	0.375 (±0.01) mM
19	TRIS	natural abundance	20 mM
20	sodium chloride	natural abundance	300 mM
21	DTT	natural abundance	5 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details FusB N150C mutant tagged with MTSL; 20 mM TrisHCl, 300 mM NaCl, pH 8.0

#	Name	Isotope labeling	Concentration
22	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
23	FusB	natural abundance	0.375 (±0.01) mM
24	TRIS	natural abundance	20 mM
25	sodium chloride	natural abundance	300 mM
26	DTT	natural abundance	5 mM

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
27	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance lysine]	0.30 (±0.01) mM
28	FusB	natural abundance	0.45 (±0.01) mM
29	TRIS	natural abundance	20 mM
30	sodium chloride	natural abundance	300 mM
31	DTT	natural abundance	5 mM

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
32	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance valine]	0.30 (±0.01) mM
33	FusB	natural abundance	0.45 (±0.01) mM
34	TRIS	natural abundance	20 mM
35	sodium chloride	natural abundance	300 mM
36	DTT	natural abundance	5 mM

Sample #8

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
37	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance phenylalanine]	0.30 (±0.01) mM
38	FusB	natural abundance	0.45 (±0.01) mM
39	TRIS	natural abundance	20 mM
40	sodium chloride	natural abundance	300 mM
41	DTT	natural abundance	5 mM

Sample #9

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
42	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance alanine]	0.30 (±0.01) mM
43	FusB	natural abundance	0.45 (±0.01) mM
44	TRIS	natural abundance	20 mM
45	sodium chloride	natural abundance	300 mM
46	DTT	natural abundance	5 mM

Sample #10

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
47	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance asparagine]	0.30 (±0.01) mM
48	FusB	natural abundance	0.45 (±0.01) mM
49	TRIS	natural abundance	20 mM
50	sodium chloride	natural abundance	300 mM
51	DTT	natural abundance	5 mM

Protein Blocks Logo

Calculated from 1 models in PDB: 2MZW, Strand ID: A, B Detail

Release date

2016-01-24

Citation

A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
Tomlinson, J.H., Thompson, G.S., Kalverda, A.P., Zhuravleva, A., O'Neill, A.
Sci. Rep. (2016), 6, 19524-19524, PubMed 26781961 , DOI 10.1038/srep19524 , Abstract

Related entities 1. EF-GC3, : 2 : 186 entities Detail

Experiments performed 24 experiments Detail

1 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
1	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
2	FusB	natural abundance	0.375 (±0.01) mM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	5 mM

2 3D HNCO

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
1	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
2	FusB	natural abundance	0.375 (±0.01) mM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	5 mM

3 3D HNCA

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
1	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
2	FusB	natural abundance	0.375 (±0.01) mM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	5 mM

4 3D HNCA

Instrument

Varian Inova - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
1	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
2	FusB	natural abundance	0.375 (±0.01) mM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	5 mM

5 3D HN(CO)CA

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
1	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
2	FusB	natural abundance	0.375 (±0.01) mM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	DTT	natural abundance	5 mM

6 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
27	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance lysine]	0.30 (±0.01) mM
28	FusB	natural abundance	0.45 (±0.01) mM
29	TRIS	natural abundance	20 mM
30	sodium chloride	natural abundance	300 mM
31	DTT	natural abundance	5 mM

7 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
32	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance valine]	0.30 (±0.01) mM
33	FusB	natural abundance	0.45 (±0.01) mM
34	TRIS	natural abundance	20 mM
35	sodium chloride	natural abundance	300 mM
36	DTT	natural abundance	5 mM

8 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
47	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance asparagine]	0.30 (±0.01) mM
48	FusB	natural abundance	0.45 (±0.01) mM
49	TRIS	natural abundance	20 mM
50	sodium chloride	natural abundance	300 mM
51	DTT	natural abundance	5 mM

9 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
37	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance phenylalanine]	0.30 (±0.01) mM
38	FusB	natural abundance	0.45 (±0.01) mM
39	TRIS	natural abundance	20 mM
40	sodium chloride	natural abundance	300 mM
41	DTT	natural abundance	5 mM

10 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
42	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance alanine]	0.30 (±0.01) mM
43	FusB	natural abundance	0.45 (±0.01) mM
44	TRIS	natural abundance	20 mM
45	sodium chloride	natural abundance	300 mM
46	DTT	natural abundance	5 mM

11 null

12 null

13 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
37	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance phenylalanine]	0.30 (±0.01) mM
38	FusB	natural abundance	0.45 (±0.01) mM
39	TRIS	natural abundance	20 mM
40	sodium chloride	natural abundance	300 mM
41	DTT	natural abundance	5 mM

14 2D 1H-15N HSQC/HMQC

Instrument

Varian Inova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details 20 mM TrisHCl, 300 mM NaCl, 5 mM DTT, pH 8.0

#	Name	Isotope labeling	Concentration
32	EF-GC3	[U-100% 15N, partial 2H (grown in D2O with 1H carbon source), 100 % natural abundance valine]	0.30 (±0.01) mM
33	FusB	natural abundance	0.45 (±0.01) mM
34	TRIS	natural abundance	20 mM
35	sodium chloride	natural abundance	300 mM
36	DTT	natural abundance	5 mM

15 null

16 null

17 null

18 null

19 2D 1H-15N HSQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details FusB R19C mutant tagged with MTSL; 20 mM TrisHCl, 300 mM NaCl, pH 8.0

#	Name	Isotope labeling	Concentration
12	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
13	FusB	natural abundance	0.375 (±0.01) mM
14	TRIS	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	DTT	natural abundance	5 mM

20 2D 1H-15N HSQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details FusB T26C mutant tagged with MTSL; 20 mM TrisHCl, 300 mM NaCl, pH 8.0

#	Name	Isotope labeling	Concentration
17	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
18	FusB	natural abundance	0.375 (±0.01) mM
19	TRIS	natural abundance	20 mM
20	sodium chloride	natural abundance	300 mM
21	DTT	natural abundance	5 mM

21 2D 1H-15N HSQC

Instrument

Varian Inova - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 273 K, pH 8.000 (±0.01), Details FusB N150C mutant tagged with MTSL; 20 mM TrisHCl, 300 mM NaCl, pH 8.0

#	Name	Isotope labeling	Concentration
22	EF-GC3	[U-100% 13C; U-100% 15N; partial 2H (grown in D2O with 1H glucose)]	0.250 (±0.01) mM
23	FusB	natural abundance	0.375 (±0.01) mM
24	TRIS	natural abundance	20 mM
25	sodium chloride	natural abundance	300 mM
26	DTT	natural abundance	5 mM

22 null

23 null

24 null

NMR combined restraints 2 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts	combined_25504_2mzw.nef
Input source #2: Coordindates	2mzw.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
2:209:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a
2:180:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
C	1	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-------410-------420-------430-------440-------450-------460-------470-------480-------490-------500
MEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQS
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQS
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-------510-------520-------530-------540-------550-------560-------570-------580-------590-------600
GGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
-------110-------120-------130-------140-------150-------160-------170-------180-------190-------200

-------610-------620-------630-------640-------650-------660-------670-------680-------690-------700
LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGELEHHHHH
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGELEHHHHH
-------210-------220-------230-------240-------250-------260-------270-------280-------290-------300

-
H
|
H
-

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

----------------------------10--------20--------30--------40--------50--------60--------70--------80
MGSSHHHHHHSSGLVPNGSHMKTMIYPHQYNYIRSVILRLKNVYKTVNDKETVKVIQSETYNDINEIFGHIDDDIEESLKVLMNIRLSNKEIEAILNKFL
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGSSHHHHHHSSGLVPNGSHMKTMIYPHQYNYIRSVILRLKNVYKTVNDKETVKVIQSETYNDINEIFGHIDDDIEESLKVLMNIRLSNKEIEAILNKFL
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--------90-------100-------110-------120-------130-------140-------150-------160-------170-------180
EYVVPFELPSPQKLQKVFKKVKKIKIPQFEEYDLKVSSFVGWNELASNRKYIIYYDEKKQLKGLYGEISNQVVKGFCTICNKESNVSLFMKKSKTNSDGQ
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
EYVVPFELPSPQKLQKVFKKVKKIKIPQFEEYDLKVSSFVGWNELASNRKYIIYYDEKKQLKGLYGEISNQVVKGFCTICNKESNVSLFMKKSKTNSDGQ
-------110-------120-------130-------140-------150-------160-------170-------180-------190-------200

-------190-------200-------210---
YVKKGDYICRDSIHCNKQLTDINQFYNFIDKLD
|||||||||||||||||||||||||||||||||
YVKKGDYICRDSIHCNKQLTDINQFYNFIDKLD
-------210-------220-------230---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	301	0	0	100.0
B	B	233	0	0	100.0

Content subtype: combined_25504_2mzw.nef

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	2		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	812		60.5 (chain: A, length: 301), 54.1 (chain: B, length: 233)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 301, Sequence coverage: 60.5%
  - Entity_assembly_ID: B, Chain length: 233, Sequence coverage: 54.1%
- Total number of assignments
  - ¹³C chemical shifts: 246
  - ¹H chemical shifts: 283
  - ¹⁵N chemical shifts: 283
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	1343	246	18.3
¹H chemical shifts	1785	157	8.8
¹⁵N chemical shifts	316	157	49.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	602	246	40.9
¹H chemical shifts	614	157	25.6
¹⁵N chemical shifts	286	157	54.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	741	0	0.0
¹H chemical shifts	1171	0	0.0
¹⁵N chemical shifts	30	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	162	0	0.0
¹H chemical shifts	162	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	129	0	0.0
¹H chemical shifts	129	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	1119	0	0.0
¹H chemical shifts	1521	126	8.3
¹⁵N chemical shifts	261	126	48.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	466	0	0.0
¹H chemical shifts	470	126	26.8
¹⁵N chemical shifts	227	126	55.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	653	0	0.0
¹H chemical shifts	1051	0	0.0
¹⁵N chemical shifts	34	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	124	0	0.0
¹H chemical shifts	124	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	131	0	0.0
¹H chemical shifts	132	0	0.0
¹⁵N chemical shifts	1	0	0.0

Covalent bonds

Saveframe: assembly
- Status: OK

Keywords EF-G, FusB, allostery, antibiotic resistance, complex, fusidic acid

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Found 1 Document (0.81 seconds)

BMRB: 25504

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Related entities 1. EF-GC3, : 2 : 186 entities Detail

Experiments performed 24 experiments Detail

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NMR combined restraints 2 contents Detail