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BMRB: 25508

2MZY

1H, 13C, and 15N Chemical Shift Assignments and structure of Probable Fe(2+)-trafficking protein from Burkholderia pseudomallei 1710b.

Authors

Tang, C., Yang, F., Barnwal, R., Barnwal, G.

Assembly

Probable Fe(2+)-trafficking protein from Burkholderia pseudomallei 1710b

Entity

1. Probable Fe(2+)-trafficking protein from Burkholderia pseudomallei 1710b (polymer, Thiol state: all free), 91 monomers, 10375.77 Da Detail

MARMIHCAKL GKEAEGLDFP PLPGELGKRL YESVSKQAWQ DWLKQQTMLI NENRLNMADP RARQYLMKQT EKYFFGEGAD QASGYVPPAQ G

Formula weight

10375.77 Da

Source organism

Burkholderia pseudomallei 1710b

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 80.4 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.4 % (878 of 1092)	74.6 % (432 of 579)	85.1 % (353 of 415)	94.9 % (93 of 98)
Backbone	93.6 % (500 of 534)	91.3 % (168 of 184)	94.0 % (249 of 265)	97.6 % (83 of 85)
Sidechain	71.5 % (458 of 641)	66.8 % (264 of 395)	79.0 % (184 of 233)	76.9 % (10 of 13)
Aromatic	46.7 % (42 of 90)	46.7 % (21 of 45)	48.8 % (21 of 43)	0.0 % (0 of 2)
Methyl	83.8 % (62 of 74)	83.8 % (31 of 37)	83.8 % (31 of 37)

1. entity

MARMIHCAKL GKEAEGLDFP PLPGELGKRL YESVSKQAWQ DWLKQQTMLI NENRLNMADP RARQYLMKQT EKYFFGEGAD QASGYVPPAQ G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Probable Fe(2+)-trafficking protein	[U-95% 13C; U-95% 15N]	1 mM
2	H2O	natural abundance	90 %
3	H2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MZY, Strand ID: A Detail

Release date

2015-03-22

Related entities 1. Probable Fe(2+)-trafficking protein from Burkholderia pseudomallei 1710b, : 1 : 1 : 103 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC