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BMRB: 25515

2N05

Solution Structure of the non-phosphorylated J-domain of Human Cysteine String Protein (CSP)

Authors

Patel, P., Lian, L., Morgan, A., Burgoyne, R.

Assembly

non-phosphorylated J-domain of Human Cysteine String Protein (CSP)

Entity

1. non-phosphorylated J-domain of Human Cysteine String Protein (CSP) (polymer, Thiol state: not present), 105 monomers, 11886.07 Da Detail

MRSPGMADQR QRSLSTSGES LYHVLGLDKN ATSDDIKKSY RKLALKYHPD KNPDNPEAAD KFKEINNAHA ILTDATKRNI YDKYGSLGLY VAEQFGEENV NTYFV

Formula weight

11886.07 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 89.0 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.0 % (1089 of 1223)	88.0 % (563 of 640)	89.4 % (421 of 471)	93.8 % (105 of 112)
Backbone	92.9 % (578 of 622)	92.5 % (196 of 212)	92.9 % (287 of 309)	94.1 % (95 of 101)
Sidechain	86.1 % (603 of 700)	85.7 % (367 of 428)	86.6 % (226 of 261)	90.9 % (10 of 11)
Aromatic	77.6 % (76 of 98)	77.6 % (38 of 49)	77.6 % (38 of 49)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. DnaJ domain of cysteine-string protein

MRSPGMADQR QRSLSTSGES LYHVLGLDKN ATSDDIKKSY RKLALKYHPD KNPDNPEAAD KFKEINNAHA ILTDATKRNI YDKYGSLGLY VAEQFGEENV NTYFV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DnaJ domain of cysteine-string protein	[U-15N]	0.5 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	DnaJ domain of cysteine-string protein	[U-13C; U-15N]	0.5 mM
7	MES	natural abundance	20 mM
8	sodium chloride	natural abundance	150 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.68 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N05, Strand ID: A Detail

Release date

2016-07-10

Citation

Phosphorylation of Cysteine String Protein Triggers a Major Conformational Switch
Patel, P., Prescott, G.R., Burgoyne, R.D., Lian, L., Morgan, A.
Structure (2016), 24, 1380-1386, PubMed 27452402 , DOI 10.1016/j.str.2016.06.009 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25514 released on 2016-07-10
Title Solution Structure of the phosphorylated J-domain of Human Cysteine String Protein (CSP)

Related entities 1. non-phosphorylated J-domain of Human Cysteine String Protein (CSP), : 1 : 8 : 33 entities Detail

Interaction partners 1. non-phosphorylated J-domain of Human Cysteine String Protein (CSP), : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC