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Chemical shift assignments and structure of the alpha-crystallin domain from human, HSPB5
Authors
Rajagopal, P., Klevit, R.E., Shi, L., Baker, D.
Assembly
alpha-crystallin domain from human, HSPB5
Entity
1. alpha-crystallin domain from human, HSPB5 (polymer, Thiol state: not present), 89 monomers, 10181.34 × 2 Da Detail

GLSEMRLEKD RFSVNLDVKH FSPEELKVKV LGDVIEVHGK HEERQDEHGF ISREFHRKYR IPADVDPLTI TSSLSSDGVL TVDGPRKQV


Total weight
20362.68 Da
Max. entity weight
10181.34 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 92.4 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.4 % (967 of 1047)94.4 % (518 of 549)88.8 % (364 of 410)96.6 % (85 of 88)
Backbone96.4 % (507 of 526)96.1 % (173 of 180)96.6 % (252 of 261)96.5 % (82 of 85)
Sidechain89.7 % (542 of 604)93.5 % (345 of 369)83.6 % (194 of 232)100.0 % (3 of 3)
Aromatic79.4 % (54 of 68)94.1 % (32 of 34)64.7 % (22 of 34)
Methyl96.9 % (93 of 96)100.0 % (48 of 48)93.8 % (45 of 48)

1. HSPB5-ACD

GLSEMRLEKD RFSVNLDVKH FSPEELKVKV LGDVIEVHGK HEERQDEHGF ISREFHRKYR IPADVDPLTI TSSLSSDGVL TVDGPRKQV

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7.5


#NameIsotope labelingTypeConcentration
1HSPB5-ACD[U-100% 13C; U-100% 15N]0.6 mM
2sodium phosphatenatural abundance50 mM
3sodium chloridenatural abundance100 mM
4EDTAnatural abundance.1 mM
5PMSFnatural abundance1 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7.5


#NameIsotope labelingTypeConcentration
6HSPB5-ACD[U-100% 13C; U-100% 15N; U-80% 2H]0.6 mM
7sodium phosphatenatural abundance50 mM
8sodium chloridenatural abundance100 mM
9EDTAnatural abundance.1 mM
10PMSFnatural abundance1 mM
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7.5, Details For measuring Residual Dipolar Coupling


#NameIsotope labelingTypeConcentration
11HSPB5-ACD[U-100% 13C; U-100% 15N; U-80% 2H]0.6 mM
12sodium phosphatenatural abundance50 mM
13sodium chloridenatural abundance100 mM
14EDTAnatural abundance.1 mM
15PMSFnatural abundance1 mM
16Pf1 phagenatural abundance14 mg/mL
Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 7.5


#NameIsotope labelingTypeConcentration
17HSPB5-ACD[U-100% 13C; U-100% 15N]1.0 mM
18sodium phosphatenatural abundance50 mM
19sodium chloridenatural abundance100 mM
20EDTAnatural abundance.1 mM
21PMSFnatural abundance1 mM
Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7.5


#NameIsotope labelingTypeConcentration
22HSPB5-ACD[U-100% 13C; U-100% 15N; 50% 2H]1.0 mM
23sodium phosphatenatural abundance50 mM
24sodium chloridenatural abundance100 mM
25EDTAnatural abundance.1 mM
26PMSFnatural abundance1 mM

LACS Plot; CA
Referencing offset: 0.4 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.4 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.24 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.91 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 9 models in PDB: 2N0K, Strand ID: A, B Detail


Release date
2015-05-31
Citation 1
A conserved histidine modulates HSPB5 structure to trigger chaperone activity in response to stress-related acidosis
Rajagopal, P., Tse, E., Borst, A.J., Delbecq, S.P., Shi, L., Dove, K.D., Baker, D., Southworth, D.R., Klevit, R.E.
Elife (2015), 4, PubMed 25962097 , DOI 10.7554/eLife.07304 ,
Citation 2
alphaB-crystallin: a hybrid solid-state/solution-state NMR investigation reveals structural aspects of the heterogeneous oligomer
Jehle, S., van Rossum, B., Stout, J.R., Noguchi, S.M., Falber, K., Rehbein, K.J., Oschkinat, H., Klevit, R.E., Rajagopal, P.J.
J. Mol. Biol. (2009), 385, 1481-1497, PubMed 19041879 , DOI 10.1016/j.jmb.2008.10.097 ,
Citation 3
Solid-state NMR and SAXS studies provide a structural basis for the activation of alphaB-crystallin oligomers
Jehle, S., Rajagopal, P., Bardiaux, B.J., Markovic, S.J., Kuhne, R., Stout, J.R., Higman, V., Klevit, R.E., van Rossum, B.J., Oschkinat, H.
Nat. Struct. Mol. Biol. (2010), 17, 1037-1042, PubMed 20802487 , DOI 10.1038/nsmb.1891 ,
Citation 4
N-terminal domain of alphaB-crystallin provides a conformational switch for multimerization and structural heterogeneity
Jehle, S., Vollmar, B., Bardiaux, B.J., Dove, K.K., Rajagopal, P., Gonen, T.J., Oschkinat, H., Klevit, R.E.
Proc. Natl. Acad. Sci. U. S. A. (2011), 108, 6409-6414, PubMed 21464278 , DOI 10.1073/pnas.1014656108 ,
Related entities 1. alpha-crystallin domain from human, HSPB5, : 1 : 8 : 203 entities Detail
Interaction partners 1. alpha-crystallin domain from human, HSPB5, : 17 interactors Detail
Experiments performed 15 experiments Detail
NMR combined restraints 5 contents Detail
Keywords ACD, Protein, crystallin, human