BMRB: 25531

N2-dG-IQ modified DNA at the G1 position of the NarI recognition sequence

Authors

Stavros, K., Hawkins, E., Rizzo, C., Stone, M.

Assembly

N2-dG IQ at G1 in NarI sequence

Entity

1. DNA (5'-D(*CP*TP*CP*(IQG)P*GP*CP*GP*CP*CP*AP*TP*C)-3') (polymer, Thiol state: not present), 12 monomers, 3858.523 Da Detail

CTCXGCGCCA TC

2. DNA (5'-D(*GP*AP*TP*GP*GP*CP*GP*CP*CP*GP*AP*G)-3') (polymer, Thiol state: not present), 12 monomers, 3791.401 Da Detail

GATGGCGCCG AG

Total weight

7649.924 Da

Max. entity weight

3858.523 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 59.6 %, Completeness (bb): 60.9 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	59.6 % (136 of 228)	59.6 % (136 of 228)
Suger, PO4	60.9 % (98 of 161)	60.9 % (98 of 161)
Nucleobase	56.7 % (38 of 67)	56.7 % (38 of 67)
Aromatic	76.1 % (35 of 46)	76.1 % (35 of 46)
Methyl	100.0 % (3 of 3)	100.0 % (3 of 3)

1. DNA (5'-D(*CP*TP*CP*(IQG)P*GP*CP*GP*CP*CP*AP*TP*C)-3')

CTCXGCGCCA TC

2. DNA (5'-D(*GP*AP*TP*GP*GP*CP*GP*CP*CP*GP*AP*G)-3')

GATGGCGCCG AG

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*CP*TP*CP*(IQG)P*GP*CP*GP*CP*CP*AP*TP*C)-3')	natural abundance		430 mM
2	DNA (5'-D(*GP*AP*TP*GP*GP*CP*GP*CP*CP*GP*AP*G)-3')	natural abundance		430 mM

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Release date

2016-03-28

Citation

Base-Displaced Intercalated Conformation of the 2-Amino-3-methylimidazo[4,5-f]quinoline N(2)-dG DNA Adduct Positioned at the Nonreiterated G(1) in the NarI Restriction Site
Stavros, K., Hawkins, E., Rizzo, C., Stone, M.
Chem. Res. Toxicol. (2015), 28, 1455-1468, PubMed 26083477 , DOI 10.1021/acs.chemrestox.5b00140 , Abstract

Experiments performed 1 experiments Detail