BMRBj - BMREntryMaster

Found 1 Document (1.133 seconds)

BMRB: 25538

2N0W

Mdmx-SJ212

Authors

Grace, C.R., Kriwacki, R.W.

Assembly

Mdmx-SJ212

Entity

1. Mdmx-SJ212, entity 1 (polymer), 89 monomers, 10148.80 Da Detail

QINQVRPKLP LLKILHAAGA QGEMFTVKEV MHYLGQYIMV KQLYDQQEQH MVYCGGDLLG ELLGRQSFSV KDPSPLYDML RKNLVTLAT

2. Mdmx-SJ212, entity 48L (non-polymer), 643.931 Da

Total weight

10792.73 Da

Max. entity weight

10148.8 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.2 % (1050 of 1080)	98.8 % (558 of 565)	94.7 % (397 of 419)	99.0 % (95 of 96)
Backbone	96.0 % (505 of 526)	98.9 % (179 of 181)	92.7 % (241 of 260)	100.0 % (85 of 85)
Sidechain	98.4 % (626 of 636)	98.7 % (379 of 384)	98.3 % (237 of 241)	90.9 % (10 of 11)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)
Methyl	95.5 % (105 of 110)	96.4 % (53 of 55)	94.5 % (52 of 55)

1. entity 1

QINQVRPKLP LLKILHAAGA QGEMFTVKEV MHYLGQYIMV KQLYDQQEQH MVYCGGDLLG ELLGRQSFSV KDPSPLYDML RKNLVTLAT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2N0W, Strand ID: A Detail

Release date

2016-01-24

Citation

Monitoring ligand-induced protein ordering in drug discovery
Grace, C., Kriwacki, R.
J. Mol. Biol. (2016), PubMed 26812210 , DOI 10.1016/j.jmb.2016.01.016 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 25377 released on 2016-01-24
    Title MDMX-P53
  BMRB: 25536 released on 2016-01-24
    Title MDMX-057
  BMRB: 25546 released on 2016-01-24
    Title MDMX-295

Related entities 1. Mdmx-SJ212, entity 1, : 1 : 24 : 9 : 83 entities Detail

Interaction partners 1. Mdmx-SJ212, entity 1, : 29 interactors Detail

More details for 29 interactors identified by 12 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

7 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

10 3D 1H-15N TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

12 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		0.5 mM
2	entity_2	natural abundance		0.6 mM

nullKeywords MDMX, negative regulators of p53