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BMRB: 25540

2N0Y

NMR structure of the complex between the C-terminal domain of the Rift Valley fever virus protein NSs and the PH domain of the Tfb1 subunit of TFIIH.

Authors

Cyr, N., de la Fuente, C., Lecoq, L., Guendel, I., Chabot, P.R., Kehn-Hall, K., Omichinski, J.G.

Assembly

NSsCT-Tfb1PH complex

Entity

1. RNA polymerase II transcription factor B subunit 1 (polymer, Thiol state: not present), 115 monomers, 12883.54 Da Detail

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. Non-structural protein NS-S (polymer, Thiol state: not present), 24 monomers, 2566.486 Da Detail

GGGGYDVEME SEEESDDDGF VEVD

Total weight

15450.026 Da

Max. entity weight

12883.54 Da

Source organism

Saccharomyces cerevisiae , Rift Valley fever virus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.3 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.3 % (1544 of 1570)	98.2 % (804 of 819)	98.5 % (597 of 606)	98.6 % (143 of 145)
Backbone	98.8 % (814 of 824)	98.9 % (280 of 283)	98.8 % (402 of 407)	98.5 % (132 of 134)
Sidechain	98.2 % (859 of 875)	97.8 % (524 of 536)	98.8 % (324 of 328)	100.0 % (11 of 11)
Aromatic	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (39 of 39)	100.0 % (1 of 1)
Methyl	98.5 % (134 of 136)	98.5 % (67 of 68)	98.5 % (67 of 68)

1. RNA polymerase II transcription factor B subunit 1

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. Non-structural protein NS-S

GGGGYDVEME SEEESDDDGF VEVD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RNA polymerase II transcription factor B subunit 1	[U-13C; U-15N]	1 mM
2	Non-structural protein NS-S	natural abundance	2 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	RNA polymerase II transcription factor B subunit 1	[U-13C; U-15N]	1 mM
6	Non-structural protein NS-S	natural abundance	2 mM
7	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	RNA polymerase II transcription factor B subunit 1	[U-15N]	0.5 mM
9	Non-structural protein NS-S	natural abundance	1 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	RNA polymerase II transcription factor B subunit 1	natural abundance	1 mM
13	Non-structural protein NS-S	[U-13C; U-15N]	0.5 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	RNA polymerase II transcription factor B subunit 1	natural abundance	0.5 mM
17	Non-structural protein NS-S	[U-13C; U-15N]	1 mM
18	D2O	natural abundance	100 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
19	RNA polymerase II transcription factor B subunit 1	natural abundance	0.4 mM
20	Non-structural protein NS-S	[U-15N]	0.2 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2N0Y, Strand ID: A, B Detail

Release date

2015-04-19

Citation

A ΩXaV motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence
Cyr, N., de la Fuente, C., Lecoq, L., Guendel, I., Chabot, P.R., Kehn-Hall, K., Omichinski, J.G.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 6021-6026, PubMed 25918396 , DOI 10.1073/pnas.1503688112 , Abstract

Related entities 1. RNA polymerase II transcription factor B subunit 1, : 2 : 4 entities Detail

Related entities 2. Non-structural protein NS-S, : 2 entities Detail

Interaction partners 1. RNA polymerase II transcription factor B subunit 1, : 49 interactors Detail

More details for 49 interactors identified by 12 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	RNA polymerase II transcription factor B subunit 1	[U-15N]	0.5 mM
9	Non-structural protein NS-S	natural abundance	1 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
19	RNA polymerase II transcription factor B subunit 1	natural abundance	0.4 mM
20	Non-structural protein NS-S	[U-15N]	0.2 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

3 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RNA polymerase II transcription factor B subunit 1	[U-13C; U-15N]	1 mM
2	Non-structural protein NS-S	natural abundance	2 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

4 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	RNA polymerase II transcription factor B subunit 1	natural abundance	1 mM
13	Non-structural protein NS-S	[U-13C; U-15N]	0.5 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RNA polymerase II transcription factor B subunit 1	[U-13C; U-15N]	1 mM
2	Non-structural protein NS-S	natural abundance	2 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

6 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	RNA polymerase II transcription factor B subunit 1	natural abundance	1 mM
13	Non-structural protein NS-S	[U-13C; U-15N]	0.5 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

7 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RNA polymerase II transcription factor B subunit 1	[U-13C; U-15N]	1 mM
2	Non-structural protein NS-S	natural abundance	2 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

8 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	RNA polymerase II transcription factor B subunit 1	natural abundance	1 mM
13	Non-structural protein NS-S	[U-13C; U-15N]	0.5 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

9 2D 1H-13C HSQC