BMRB: 25551

Backbone amide chemical shift assignments of the CN-bound yeast cytochrome c peroxidase covalently cross-linked to yeast iso-1 cytochrome c

Authors

Volkov, A., Van de Water, K.

Assembly

Covalent, disulfide crosslink of V197C/C128A yeast cytochrome c peroxidase and A81C/C102T yeast iso-1 cytochrome c

Entity

1. CcP (polymer, Thiol state: all disulfide bound), 294 monomers, 33503.85 Da Detail

TTPLVHVASV EKGRSYEDFQ KVYNAIALKL REDDEYDNYI GYGPVLVRLA WHTSGTWDKH DNTGGSYGGT YRFKKEFNDP SNAGLQNGFK FLEPIHKEFP WISSGDLFSL GGVTAVQEMQ GPKIPWRAGR VDTPEDTTPD NGRLPDADKD ADYVRTFFQR LNMNDREVVA LMGAHALGKT HLKNSGYEGP WGAANNCFTN EFYLNLLNED WKLEKNDANN EQWDSKSGYM MLPTDYSLIQ DPKYLSIVKE YANDQDKFFK DFSKAFEKLL ENGITFPKDA PSPFIFKTLE EQGL

2. Cc (polymer, Thiol state: free and disulfide bound), 108 monomers, 12050.64 Da Detail

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMCFGGL KKEKDRNDLI TYLKKATE

3. Cc1 (polymer, Thiol state: all free), 108 monomers, 12050.64 Da Detail

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKACE

4. PROTOPORPHYRIN IX CONTAINING FE (non-polymer), 616.487 Da
5. HEME C (non-polymer), 618.503 × 2 Da

Total weight

59458.63 Da

Max. entity weight

33503.85 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS197:SG	2:CYS86:SG

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 47.1 %, Completeness: 15.4 %, Completeness (bb): 32.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	15.4 % (570 of 3700)	10.5 % (331 of 3158)	44.1 % (239 of 542)
Backbone	32.6 % (499 of 1530)	25.0 % (261 of 1044)	49.0 % (238 of 486)
Sidechain	3.3 % (71 of 2170)	3.3 % (70 of 2114)	1.8 % (1 of 56)
Aromatic	0.0 % (0 of 317)	0.0 % (0 of 308)	0.0 % (0 of 9)
Methyl	5.0 % (11 of 218)	5.0 % (11 of 218)

1. CcP

TTPLVHVASV EKGRSYEDFQ KVYNAIALKL REDDEYDNYI GYGPVLVRLA WHTSGTWDKH DNTGGSYGGT YRFKKEFNDP SNAGLQNGFK FLEPIHKEFP WISSGDLFSL GGVTAVQEMQ GPKIPWRAGR VDTPEDTTPD NGRLPDADKD ADYVRTFFQR LNMNDREVVA LMGAHALGKT HLKNSGYEGP WGAANNCFTN EFYLNLLNED WKLEKNDANN EQWDSKSGYM MLPTDYSLIQ DPKYLSIVKE YANDQDKFFK DFSKAFEKLL ENGITFPKDA PSPFIFKTLE EQGL

2. Cc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMCFGGL KKEKDRNDLI TYLKKATE

3. Cc1

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKACE

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	CcP	[U-2H; U-15N]	protein	0.4 mM
2	Cc	natural abundance	protein	0.4 mM
3	Cc1	natural abundance	protein	0.4 mM
4	sodium phosphate	natural abundance	buffer	20 mM
5	H2O	natural abundance	solvent	93 %
6	D2O	[U-2H]	solvent	7 %

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Protein Blocks Logo

Calculated from 15 models in PDB: 2N18, Strand ID: A, B, C Detail

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Release date

2015-05-07

Citation

The low-affinity complex of cytochrome c and its peroxidase
Van de Water, K., Sterckx, Y.G.J., Volkov, A.N.
Nat. Commun. (2015), 6, 7073-7073, PubMed 25944250 , DOI 10.1038/ncomms8073 , Abstract

Related entities 1. CcP, : 1 : 1 : 72 : 101 entities Detail

Related entities 2. Cc, : 1 : 35 entities Detail

Related entities 3. Cc1, : 1 : 36 entities Detail

Interaction partners 1. CcP, : 14 interactors Detail

Experiments performed 1 experiments Detail