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BMRB: 25552

2N19

STIL binding to Polo-box 3 of PLK4 regulates centriole duplication - NMR solution structure of human Polo-box 3

Authors

Boehm, R., Arquint, C., Gabryjonczyk, A., Imseng, S., Sauer, E., Nigg, E., Maier, T., Hiller, S.

Assembly

PLK4-PB3 monomer

Entity

1. PLK4-PB3 monomer (polymer, Thiol state: all free), 88 monomers, 9622.769 Da Detail

GSAQLLKSVF VKNVGWATQL TSGAVWVQFN DGSQLVVQAG VSSISYTSPN GQTTRYGENE KLPDYIKQKL QCLSSILLMF SNPTPNFH

Formula weight

9622.769 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.0 %, Completeness: 47.3 %, Completeness (bb): 75.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.3 % (485 of 1025)	25.3 % (134 of 529)	68.2 % (270 of 396)	81.0 % (81 of 100)
Backbone	75.8 % (394 of 520)	47.5 % (85 of 179)	89.5 % (230 of 257)	94.0 % (79 of 84)
Sidechain	28.0 % (164 of 586)	14.0 % (49 of 350)	51.4 % (113 of 220)	12.5 % (2 of 16)
Aromatic	4.3 % (4 of 92)	4.3 % (2 of 46)	0.0 % (0 of 44)	100.0 % (2 of 2)
Methyl	79.0 % (79 of 100)	76.0 % (38 of 50)	82.0 % (41 of 50)

1. entity

GSAQLLKSVF VKNVGWATQL TSGAVWVQFN DGSQLVVQAG VSSISYTSPN GQTTRYGENE KLPDYIKQKL QCLSSILLMF SNPTPNFH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.15 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	PLK4-PB3	[U-2H; U-13C; U-15N]	protein	0.6 ~ 1.2 uM
2	MOPS	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	30 mM
4	TCEP	natural abundance		2 mM
5	H2O	natural abundance	solvent	95 %
6	D2O	[U-2H]	solvent	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.97 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.97 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.68 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N19, Strand ID: A Detail

Release date

2015-08-06

Citation

STIL binding to Polo-box 3 of PLK4 regulates centriole duplication
Arquint, C., Gabryjonczyk, A., Imseng, S., Boehm, R., Sauer, E., Hiller, S., Nigg, E., Maier, T.
Elife (2015), 4, PubMed 26188084 , DOI 10.7554/eLife.07888 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 26547 released on 2015-08-06
Title STIL binding to the Polo-box 3 of PLK4 regulates centriole duplication - Backbone assignment of human Polo-box 3 bound to STIL

Related entities 1. PLK4-PB3 monomer, : 1 : 2 : 3 : 27 entities Detail

Interaction partners 1. PLK4-PB3 monomer, : 25 interactors Detail

More details for 25 interactors identified by 11 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC