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BMRB: 25554

2N1B

NMR solution structure of nucleotide-free Ran GTPase

Authors

Bacot-Davis, V.R., Palmenberg, A.C.

Assembly

nucleotide-free Ran GTPase

Entity

1. nucleotide-free Ran GTPase (polymer, Thiol state: all free), 216 monomers, 24422.78 Da Detail

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Formula weight

24422.78 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 86.4 %, Completeness (bb): 86.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.4 % (2225 of 2575)	83.8 % (1121 of 1337)	86.9 % (879 of 1012)	99.6 % (225 of 226)
Backbone	86.6 % (1101 of 1272)	64.3 % (279 of 434)	97.6 % (619 of 634)	99.5 % (203 of 204)
Sidechain	87.8 % (1322 of 1505)	93.2 % (842 of 903)	79.0 % (458 of 580)	100.0 % (22 of 22)
Aromatic	97.9 % (229 of 234)	100.0 % (117 of 117)	95.6 % (109 of 114)	100.0 % (3 of 3)
Methyl	97.7 % (250 of 256)	96.9 % (124 of 128)	98.4 % (126 of 128)

1. entity

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2N1B, Strand ID: A Detail

Release date

2016-04-17

Citation

Nuclear Magnetic Resonance Structure of Ran GTPase Determines C-terminal Tail Conformational Dynamics
Bacot-Davis, V.R., Palmenberg, A.C.
J. Biomol. NMR

Related entities 1. nucleotide-free Ran GTPase, : 1 : 70 : 1 : 32 : 91 entities Detail

Interaction partners 1. nucleotide-free Ran GTPase, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

4 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

5 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

6 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

7 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

10 3D HNCACB

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

11 31P NMR

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Dihedral angle restraints	combined_25554_2n1b.nef
Input source #2: Coordindates	2n1b.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-------------------------------------------------10--------20--------30--------40--------50--------6
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSRSAAGTMAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSRSAAGTMAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPI
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

0--------70--------80--------90-------100-------110-------120-------130-------140-------150-------16
KFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
KFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEK
-------110-------120-------130-------140-------150-------160-------170-------180-------190-------200

0-------170-------180-------190-------200-------210-------220
PFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDLEFEA
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
PFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDLEFEA
-------210-------220-------230-------240-------250-------260-

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	261	0	0	100.0

Content subtype: combined_25554_2n1b.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	2341		82.4 (chain: A, length: 261)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_2	OK	264 (264)	.	77.4 (chain: A, length: 261)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 261, Sequence coverage: 82.4%

-------------------------------------------------10--------20--------30--------40--------50--------6
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSRSAAGTMAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPI
                                         |||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
.........................................MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYV.TLGVEVHPLVFHTNRGPI
-------------------------------------------------10--------20--------30--------40--------50--------6

0--------70--------80--------90-------100-------110-------120-------130-------140-------150-------16
KFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
KFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEK
0--------70--------80--------90-------100-------110-------120-------130-------140-------150-------16

0-------170-------180-------190-------200-------210-------220
PFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDLEFEA
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||    
PFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL    
0-------170-------180-------190-------200-------210------

Total number of assignments
- ¹H chemical shifts: 1173
- ¹³C chemical shifts: 874
- ¹⁵N chemical shifts: 293

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
7	PRO	N	118.677
12	LYS	HZ1	7.433
12	LYS	HZ2	7.433
12	LYS	HZ3	7.433
12	LYS	NZ	49.231
21	THR	HG1	6.377
23	LYS	HZ1	7.433
23	LYS	HZ2	7.433
23	LYS	HZ3	7.433
23	LYS	NZ	30.294
24	THR	HG1	5.907
25	THR	HG1	5.389
28	LYS	HZ1	7.501
28	LYS	HZ2	7.501
28	LYS	HZ3	7.501
28	LYS	NZ	39.203
29	ARG	HH11	6.69
29	ARG	HH12	6.621
29	ARG	HH21	6.768
29	ARG	HH22	6.915
29	ARG	CZ	161.621
29	ARG	NH1	79.392
29	ARG	NH2	79.302
30	HIS	HD1	10.269
30	HIS	HE2	11.376
30	HIS	ND1	194.294
30	HIS	NE2	177.332
32	THR	HG1	5.712
37	LYS	HZ1	7.247
37	LYS	HZ2	7.247
37	LYS	HZ3	7.247
37	LYS	NZ	53.201
38	LYS	HZ1	7.364
38	LYS	HZ2	7.364
38	LYS	HZ3	7.364
38	LYS	NZ	52.396
39	TYR	HH	9.193
39	TYR	CZ	155.174
42	THR	HG1	5.311
48	HIS	HD1	10.435
48	HIS	HE2	11.298
48	HIS	ND1	194.229
48	HIS	NE2	178.938
49	PRO	N	123.193
53	HIS	HD1	10.367
53	HIS	HE2	11.083
53	HIS	ND1	197.322
53	HIS	NE2	177.382
54	THR	HG1	5.594
56	ARG	HH11	6.797
56	ARG	HH12	6.758
56	ARG	HH21	6.885
56	ARG	HH22	6.983
56	ARG	CZ	161.914
56	ARG	NH1	81.938
56	ARG	NH2	81.229
58	PRO	N	114.56
60	LYS	HZ1	7.531
60	LYS	HZ2	7.531
60	LYS	HZ3	7.531
60	LYS	NZ	48.294
64	TRP	CD2	128.509
64	TRP	CE2	139.058
66	THR	HG1	6.015
71	LYS	HZ1	7.511
71	LYS	HZ2	7.511
71	LYS	HZ3	7.511
71	LYS	NZ	49.002
76	ARG	HH11	6.778
76	ARG	HH12	6.748
76	ARG	HH21	6.875
76	ARG	HH22	6.905
76	ARG	CZ	159.57
76	ARG	NH1	81.395
76	ARG	NH2	79.394
79	TYR	HH	9.203
79	TYR	CZ	154.881
80	TYR	HH	9.213
80	TYR	CZ	155.467
84	GLN	CD	178.91
93	THR	HG1	5.761
95	ARG	HH11	6.748
95	ARG	HH12	6.719
95	ARG	HH21	6.827
95	ARG	HH22	6.934
95	ARG	CZ	158.984
95	ARG	NH1	77.483
95	ARG	NH2	77.294
97	THR	HG1	5.477
98	TYR	HH	9.193
98	TYR	CZ	154.295
99	LYS	HZ1	7.73
99	LYS	HZ2	7.73
99	LYS	HZ3	7.73
99	LYS	NZ	42.443
102	PRO	N	117.084
105	HIS	HD1	10.396
105	HIS	HE2	11.112
105	HIS	ND1	192.113
105	HIS	NE2	179.221
106	ARG	HH11	6.797
106	ARG	HH12	6.729
106	ARG	HH21	6.866
106	ARG	HH22	6.905
106	ARG	CZ	159.863
106	ARG	NH1	79.729
106	ARG	NH2	79.294
110	ARG	HH11	6.719
110	ARG	HH12	6.748
110	ARG	HH21	6.885
110	ARG	HH22	6.827
110	ARG	CZ	158.984
110	ARG	NH1	80.302
110	ARG	NH2	80.394
116	PRO	N	117.548
123	LYS	HZ1	7.306
123	LYS	HZ2	7.306
123	LYS	HZ3	7.306
123	LYS	NZ	49.293
127	LYS	HZ1	7.511
127	LYS	HZ2	7.511
127	LYS	HZ3	7.511
127	LYS	NZ	47.334
129	ARG	HH11	6.846
129	ARG	HH12	6.797
129	ARG	HH21	6.885
129	ARG	HH22	6.905
129	ARG	CZ	162.793
129	ARG	NH1	80.284
129	ARG	NH2	80.393
130	LYS	HZ1	7.668
130	LYS	HZ2	7.668
130	LYS	HZ3	7.668
130	LYS	NZ	42.394
132	LYS	HZ1	7.228
132	LYS	HZ2	7.228
132	LYS	HZ3	7.228
132	LYS	NZ	49.592
134	LYS	HZ1	7.404
134	LYS	HZ2	7.404
134	LYS	HZ3	7.404
134	LYS	NZ	49.302
139	HIS	HD1	10.687
139	HIS	HE2	11.493
139	HIS	ND1	204.849
139	HIS	NE2	189.303
140	ARG	HH11	6.778
140	ARG	HH12	6.739
140	ARG	HH21	6.885
140	ARG	HH22	6.963
140	ARG	CZ	160.742
140	ARG	NH1	78.484
140	ARG	NH2	78.393
141	LYS	HZ1	7.306
141	LYS	HZ2	7.306
141	LYS	HZ3	7.306
141	LYS	NZ	49.238
142	LYS	HZ1	7.355
142	LYS	HZ2	7.355
142	LYS	HZ3	7.355
142	LYS	NZ	49.223
146	TYR	HH	9.213
146	TYR	CZ	154.881
147	TYR	HH	9.291
147	TYR	CZ	154.588
152	LYS	HZ1	7.462
152	LYS	HZ2	7.462
152	LYS	HZ3	7.462
152	LYS	NZ	49.22
155	TYR	HH	9.183
155	TYR	CZ	155.467
159	LYS	HZ1	7.364
159	LYS	HZ2	7.364
159	LYS	HZ3	7.364
159	LYS	NZ	42.592
160	PRO	N	113.099
163	TRP	CD2	128.509
163	TRP	CE2	138.472
166	ARG	HH11	6.797
166	ARG	HH12	6.748
166	ARG	HH21	6.875
166	ARG	HH22	6.915
166	ARG	CZ	159.57
166	ARG	NH1	77.827
166	ARG	NH2	77.392
167	LYS	HZ1	7.316
167	LYS	HZ2	7.316
167	LYS	HZ3	7.316
167	LYS	NZ	46.773
172	PRO	N	120.802
180	PRO	N	112.236
184	PRO	N	117.482
185	PRO	N	116.22
186	GLU	CD	178.91
191	PRO	N	121.4
196	GLN	CD	178.617
197	TYR	HH	9.222
197	TYR	CZ	156.346
199	HIS	HD1	10.494
199	HIS	HE2	11.181
199	HIS	ND1	198.848
199	HIS	NE2	182.872
206	THR	HG1	5.956
207	THR	HG1	5.986
210	PRO	N	113.896

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1579	1052	66.6
¹³C chemical shifts	1190	850	71.4
¹⁵N chemical shifts	284	233	82.0

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	529	231	43.7
¹³C chemical shifts	522	421	80.7
¹⁵N chemical shifts	248	203	81.9

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1050	821	78.2
¹³C chemical shifts	668	429	64.2
¹⁵N chemical shifts	36	30	83.3

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	144	124	86.1
¹³C chemical shifts	144	126	87.5

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	138	117	84.8
¹³C chemical shifts	135	109	80.7
¹⁵N chemical shifts	3	3	100.0

Redox state of CYS
Peptide bond of PRO
Tautomeric state of HIS
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: A

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 261, Sequence coverage: 77.4%
- Total number of restraints: 264
- Total number of restraints per polymer type: 264
- Weight of restraints: 1.0 (264)
- Potential type of restraints: square-well-parabolic (264)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords CYTOPLASM, EDTA, G PROTEIN, GTP-BINDING, NUCLEOTIDE-BINDING, NUCLEUS, PROTEIN TRANSPORT, TRANSPORT PROTEIN