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BMRB: 25559

2N1G

Structure of C-terminal domain of human polymerase Rev1 in complex with PolD3 RIR-motif

Authors

Pustovalova, Y., Korzhnev, D.

Assembly

C-terminal domain of human polymerase Rev1 in complex with PolD3 RIR-motif

Entity

1. Rev1-CT (polymer, Thiol state: all free), 94 monomers, 10945.54 Da Detail

NLAGAVEFND VKTLLREWIT TISDPMEEDI LQVVKYCTDL IEEKDLEKLD LVIKYMKRLM QQSVESVWNM AFDFILDNVQ VVLQQTYGST LKVT

2. PolD3 (polymer, Thiol state: not present), 16 monomers, 1776.110 Da Detail

KGNMMSNFFG KAAMNK

Total weight

12721.65 Da

Max. entity weight

10945.54 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.5 %, Completeness: 76.7 %, Completeness (bb): 72.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.7 % (1026 of 1337)	82.7 % (573 of 693)	67.3 % (350 of 520)	83.1 % (103 of 124)
Backbone	72.3 % (476 of 658)	83.0 % (185 of 223)	61.3 % (200 of 326)	83.5 % (91 of 109)
Sidechain	81.8 % (642 of 785)	82.6 % (388 of 470)	80.7 % (242 of 300)	80.0 % (12 of 15)
Aromatic	51.0 % (50 of 98)	61.2 % (30 of 49)	38.3 % (18 of 47)	100.0 % (2 of 2)
Methyl	96.4 % (135 of 140)	95.7 % (67 of 70)	97.1 % (68 of 70)

1. Rev1-CT

NLAGAVEFND VKTLLREWIT TISDPMEEDI LQVVKYCTDL IEEKDLEKLD LVIKYMKRLM QQSVESVWNM AFDFILDNVQ VVLQQTYGST LKVT

2. PolD3

KGNMMSNFFG KAAMNK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 273 K, pH 7.0, Details a 15N/13C-labeled hRev1-CT with excess of an unlabeled PolD3 peptide

#	Name	Isotope labeling	Concentration
1	Rev1-CT	[U-100% 13C; U-100% 15N]	0.5 mM
2	PolD3	natural abundance	0.5 ~ 0.8 mM
3	potassium phosphate	natural abundance	50 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	2 mM
6	EDTA	natural abundance	0.5 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.868 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.868 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.868 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N1G, Strand ID: A, B Detail

Release date

2016-04-11

Citation

Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis
Pustovalova, Y., Magalhaes, M.T.Q., D'Souza, S., Rizzo, A.A., Korza, G., Walker, G.C., Korzhnev, D.M.
Biochemistry (2016), 55, 2043-2053, PubMed 26982350 , DOI 10.1021/acs.biochem.5b01282 , Abstract

Related entities 1. Rev1-CT, : 3 : 3 : 4 entities Detail

Related entities 2. PolD3, : 1 : 2 : 1 entities Detail

Interaction partners 1. Rev1-CT, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC