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BMRB: 25562

2N1I

Structure of the PR domain from PRDM16

Authors

Sun, Y., Armstrong, G., Briknarova, K.

Assembly

PR domain from PRDM16

Entity

1. PR domain from PRDM16 (polymer, Thiol state: all free), 176 monomers, 19153.38 Da Detail

GSGFPTSEDF TPKEGSPYEA PVYIPEDIPI PADFELRESS IPGAGLGVWA KRKMEAGERL GPCVVVPRAA AKETDFGWEQ ILTDVEVSPQ EGCITKISED LGSEKFCVDA NQAGAGSWLK YIRVACSCDD QNLTMCQISE QIYYKVIKDI EPGEELLVHV KEGVYPLGTV PPGLDE

Formula weight

19153.38 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.7 % (1899 of 1984)	95.8 % (988 of 1031)	95.1 % (743 of 781)	97.7 % (168 of 172)
Backbone	97.7 % (1002 of 1026)	98.3 % (348 of 354)	97.3 % (497 of 511)	97.5 % (157 of 161)
Sidechain	94.4 % (1055 of 1117)	94.5 % (640 of 677)	94.2 % (404 of 429)	100.0 % (11 of 11)
Aromatic	87.0 % (120 of 138)	87.0 % (60 of 69)	86.4 % (57 of 66)	100.0 % (3 of 3)
Methyl	92.5 % (172 of 186)	93.5 % (87 of 93)	91.4 % (85 of 93)

1. entity

GSGFPTSEDF TPKEGSPYEA PVYIPEDIPI PADFELRESS IPGAGLGVWA KRKMEAGERL GPCVVVPRAA AKETDFGWEQ ILTDVEVSPQ EGCITKISED LGSEKFCVDA NQAGAGSWLK YIRVACSCDD QNLTMCQISE QIYYKVIKDI EPGEELLVHV KEGVYPLGTV PPGLDE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	0.45-0.55 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	50 mM
4	DTT	natural abundance	2.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	entity	[U-99% 15N]	0.4-0.5 mM
8	potassium phosphate	natural abundance	20 mM
9	potassium chloride	natural abundance	50 mM
10	DTT	natural abundance	2.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	entity	natural abundance	0.5 mM
14	potassium phosphate	natural abundance	20 mM
15	potassium chloride	natural abundance	50 mM
16	DTT	natural abundance	2.5 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.755 ppm	internal	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	4.755 ppm	internal	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N1I, Strand ID: A Detail

Release date

2016-07-04

Citation

Structure of the PR Domain from PRDM16
Sun, Y., Armstrong, G., Briknarova, K.
Not known

Related entities 1. PR domain from PRDM16, : 1 : 2 : 26 entities Detail

Interaction partners 1. PR domain from PRDM16, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC