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BMRB: 25573

2N1R

NMR Structure of the Myristylated Feline Immunodeficiency Virus Matrix Protein

Authors

Brown, L.A., Cox, C., Button, R.J., Baptiste, J., Bahlow, K., Spurrier, V., Luttge, B.G., Kuo, L., Freed, E.O., Summers, M.F., Kyser, J., Summers, H.R.

Assembly

Myristylated Feline Immunodeficiency Virus Matrix Protein

Entity

1. Myristylated Feline Immunodeficiency Virus Matrix Protein (polymer), 135 monomers, 14709.82 Da Detail

XGNGASRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPS MKEAGGKEEG PPQAY

Formula weight

14709.82 Da

Source organism

Felis catus

Exptl. method

solution NMR

Refine. method

CYANA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 64.3 %, Completeness (bb): 79.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.3 % (967 of 1505)	62.6 % (493 of 787)	60.7 % (350 of 577)	87.9 % (124 of 141)
Backbone	79.0 % (627 of 794)	91.4 % (254 of 278)	65.1 % (252 of 387)	93.8 % (121 of 129)
Sidechain	53.9 % (447 of 830)	47.0 % (239 of 509)	66.3 % (205 of 309)	25.0 % (3 of 12)
Aromatic	41.2 % (33 of 80)	40.0 % (16 of 40)	39.5 % (15 of 38)	100.0 % (2 of 2)
Methyl	81.8 % (108 of 132)	78.8 % (52 of 66)	84.8 % (56 of 66)

1. Feline Immunodeficiency Virus Matrix Protein

XGNGASRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPS MKEAGGKEEG PPQAY

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	150 mM
3	DTT	natural abundance	10 mM
4	ammonium chloride	[U-99% 15N]	1 g/L
5	glucose	natural abundance	4 g/L
6	MYRISTIC ACID	natural abundance	0.5 mg/mL
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	Feline Immunodeficiency Virus Matrix Protein	[U-90% 15N]	0.0 ~ 0.0 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT

#	Name	Isotope labeling	Concentration
10	sodium phosphate	natural abundance	50 mM
11	sodium chloride	natural abundance	150 mM
12	DTT	natural abundance	10 mM
13	glucose	[U-100% 13C]	4 g/L
14	ammonium chloride	natural abundance	1 g/L
15	MYRISTIC ACID	natural abundance	0.5 mg/mL
16	D2O	natural abundance	100 %
17	Feline Immunodeficiency Virus Matrix Protein	[U-100% 13C]	0.0 ~ 0.0 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT

#	Name	Isotope labeling	Concentration
18	sodium phosphate	natural abundance	50 mM
19	sodium chloride	natural abundance	150 mM
20	DTT	natural abundance	10 mM
21	glucose	[U-100% 13C]	4 g/L
22	ammonium chloride	natural abundance	1 g/L
23	MYRISTIC ACID	natural abundance	0.5 mg/mL
24	D2O	natural abundance	100 %
25	Feline Immunodeficiency Virus Matrix Protein	[U-100% 13C]	0.0 ~ 0.0 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT

#	Name	Isotope labeling	Concentration
26	sodium phosphate	natural abundance	50 mM
27	sodium chloride	natural abundance	150 mM
28	DTT	natural abundance	10 mM
29	glucose	[U-100% 13C]	4 g/L
30	ammonium chloride	natural abundance	1 g/L
31	MYRISTIC ACID	natural abundance	0.5 mg/mL
32	D2O	natural abundance	100 %
33	Feline Immunodeficiency Virus Matrix Protein	[U-100% 13C]	0.0 ~ 0.0 mM

Sample #5

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT

#	Name	Isotope labeling	Concentration
34	sodium phosphate	natural abundance	50 mM
35	sodium chloride	natural abundance	150 mM
36	DTT	natural abundance	10 mM
37	ammonium chloride	[U-99% 15N]	1 g/L
38	glucose	natural abundance	4 g/L
39	MYRISTIC ACID	natural abundance	0.5 mg/mL
40	H2O	natural abundance	90 %
41	D2O	natural abundance	10 %
42	Feline Immunodeficiency Virus Matrix Protein	[U-90% 15N]	0.0 ~ 0.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2N1R, Strand ID: A Detail

Release date

2015-05-25

Citation

NMR structure of the myristylated feline immunodeficiency virus matrix protein
Brown, L.A., Cox, C., Baptiste, J., Summers, H.R., Button, R.J., Bahlow, K., Spurrier, V., Kyser, J., Luttge, B.G., Kuo, L., Freed, E.O., Summers, M.F.
Viruses (2015), 7, 2210-2229, PubMed 25941825 , DOI 10.3390/v7052210 , Abstract

Related entities 1. Myristylated Feline Immunodeficiency Virus Matrix Protein, : 1 : 5 : 4 entities Detail

Experiments performed 11 experiments Detail

1 15N-1HN HSQC