BMRBj - BMREntryMaster

Found 1 Document (0.762 seconds)

BMRB: 25575

2N1U

Structure of SAP30L corepressor protein

Authors

Tossavainen, H., Permi, P.

Assembly

SAP30L corepressor protein

Entity

1. SAP30L corepressor protein, entity 1 (polymer, Thiol state: free and other bound), 70 monomers, 8101.297 Da Detail

GSYGQSCCLI EDGERCVRPA GNASFSKRVQ KSISQKKLKL DIDKSVRHLY ICDFHKNFIQ SVRNKRKRKT

2. ZINC ION (non-polymer), 65.409 Da

Total weight

8166.706 Da

Max. entity weight

8101.297 Da

Entity Connection

metal coordination 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS7:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS16:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS52:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.8 %, Completeness (bb): 82.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.8 % (749 of 853)	94.1 % (429 of 456)	79.8 % (256 of 321)	84.2 % (64 of 76)
Backbone	82.3 % (344 of 418)	95.8 % (137 of 143)	69.4 % (143 of 206)	92.8 % (64 of 69)
Sidechain	93.8 % (470 of 501)	93.3 % (292 of 313)	98.3 % (178 of 181)	0.0 % (0 of 7)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. entity 1

GSYGQSCCLI EDGERCVRPA GNASFSKRVQ KSISQKKLKL DIDKSVRHLY ICDFHKNFIQ SVRNKRKRKT

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	0.5 mM
2	ZINC ION	natural abundance	0.5 mM
3	BIS-TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	30 mM
5	sodium azide	natural abundance	0.04 %
6	H2O	natural abundance	93 %
7	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.76 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.76 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.76 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2N1U, Strand ID: A Detail

Release date

2015-11-22

Citation

Redox-dependent disulfide bond formation in SAP30L corepressor protein: Implications for structure and function
Laitaoja, M., Tossavainen, H., Pihlajamaa, T., Valjakka, J., Viiri, K., Lohi, O., Permi, P., Janis, J.
Protein Sci. (2015), 25, 572-586, PubMed 26609676 , DOI 10.1002/pro.2849 , Abstract

Related entities 1. SAP30L corepressor protein, entity 1, : 1 : 5 : 19 entities Detail

Interaction partners 1. SAP30L corepressor protein, entity 1, : 9 interactors Detail

More details for 9 interactors identified by 6 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC