BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	87.1 % (1136 of 1304)	86.2 % (594 of 689)	87.8 % (437 of 498)	89.7 % (105 of 117)
Backbone	87.1 % (573 of 658)	85.9 % (195 of 227)	87.0 % (282 of 324)	89.7 % (96 of 107)
Sidechain	87.3 % (653 of 748)	86.4 % (399 of 462)	88.8 % (245 of 276)	90.0 % (9 of 10)
Aromatic	68.9 % (62 of 90)	68.9 % (31 of 45)	68.9 % (31 of 45)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. SUMO1

MGSSHHHHHH SQDPMSDQEA KPSTEDLGDK KEGEYIKLKV IGQDSSEIHF KVKMTTHLKK LKESYCQRQG VPMNSLRFLF EGQRIADNHT PKELGMEEED VIEVYQEQTG G

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 15N SUMO1

#	Name	Isotope labeling	Concentration
9	SUMO1	[U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
10	potassium phosphate	natural abundance	10 (±1.0) mM
11	potassium chloride	natural abundance	100 (±1.0) mM
12	DTT	natural abundance	2 (±0.05) mM
13	EDTA	natural abundance	0.1 (±0.005) mM
14	sodium azide	natural abundance	0.001 (±0.001) %
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 15N SUMO1 in Pf1 phage alignment medium.

#	Name	Isotope labeling	Concentration
17	SUMO1	[U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
18	potassium phosphate	natural abundance	10 (±1.0) mM
19	potassium chloride	natural abundance	100 (±1.0) mM
20	DTT	natural abundance	2 (±0.05) mM
21	EDTA	natural abundance	0.1 (±0.005) mM
22	sodium azide	natural abundance	0.001 (±0.001) %
23	Pf1 phage	natural abundance	10 (±0.1) w/v
24	H2O	natural abundance	90 %
25	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details SUMO1

#	Name	Isotope labeling	Concentration
26	SUMO1	natural abundance	0.5 ~ 1.0 (±0.05) mM
27	potassium phosphate	natural abundance	10 (±1.0) mM
28	potassium chloride	natural abundance	100 (±1.0) mM
29	DTT	natural abundance	2 (±0.05) mM
30	EDTA	natural abundance	0.1 (±0.005) mM
31	sodium azide	natural abundance	0.001 (±0.001) %
32	H2O	natural abundance	90 %
33	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N1V, Strand ID: A Detail

Release date

2016-04-17

Citation

Structures of human SUMO
Naik, M.T., Naik, N., Shih, H., Huang, T.
Not known

Related entities 1. human SUMO1, : 1 : 27 : 41 entities Detail

Interaction partners 1. human SUMO1, : 123 interactors Detail

More details for 123 interactors identified by 62 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 15N SUMO1

#	Name	Isotope labeling	Concentration
9	SUMO1	[U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
10	potassium phosphate	natural abundance	10 (±1.0) mM
11	potassium chloride	natural abundance	100 (±1.0) mM
12	DTT	natural abundance	2 (±0.05) mM
13	EDTA	natural abundance	0.1 (±0.005) mM
14	sodium azide	natural abundance	0.001 (±0.001) %
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

9 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

10 3D HBHAcoNH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

11 2D-hbCBcgcdHD

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

12 2D-hbCBcgcdceHE

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

13 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

14 2D 1H-1H NOESY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details SUMO1

#	Name	Isotope labeling	Concentration
26	SUMO1	natural abundance	0.5 ~ 1.0 (±0.05) mM
27	potassium phosphate	natural abundance	10 (±1.0) mM
28	potassium chloride	natural abundance	100 (±1.0) mM
29	DTT	natural abundance	2 (±0.05) mM
30	EDTA	natural abundance	0.1 (±0.005) mM
31	sodium azide	natural abundance	0.001 (±0.001) %
32	H2O	natural abundance	90 %
33	D2O	natural abundance	10 %

15 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

16 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 13C, 15N SUMO1

#	Name	Isotope labeling	Concentration
1	SUMO1	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
2	potassium phosphate	natural abundance	10 (±1.0) mM
3	potassium chloride	natural abundance	100 (±1.0) mM
4	DTT	natural abundance	2 (±0.05) mM
5	EDTA	natural abundance	0.1 (±0.005) mM
6	sodium azide	natural abundance	0.001 (±0.001) %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

17 2D 1H-15N HSQC IPAP

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.1), Details 15N SUMO1 in Pf1 phage alignment medium.

#	Name	Isotope labeling	Concentration
17	SUMO1	[U-100% 15N]	0.5 ~ 1.0 (±0.05) mM
18	potassium phosphate	natural abundance	10 (±1.0) mM
19	potassium chloride	natural abundance	100 (±1.0) mM
20	DTT	natural abundance	2 (±0.05) mM
21	EDTA	natural abundance	0.1 (±0.005) mM
22	sodium azide	natural abundance	0.001 (±0.001) %
23	Pf1 phage	natural abundance	10 (±0.1) w/v
24	H2O	natural abundance	90 %
25	D2O	natural abundance	10 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_25576_2n1v.nef
Input source #2: Coordindates	2n1v.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

----------------------10--------20--------30--------40--------50--------60--------70--------80------
MGSSHHHHHHSQDPMSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEED
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGSSHHHHHHSQDPMSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEED
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--90-------
VIEVYQEQTGG
|||||||||||
VIEVYQEQTGG
-------110-

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	111	0	0	100.0

Content subtype: combined_25576_2n1v.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts	Warning	1144		87.4 (chain: A, length: 111)
1	Distance restraints	CNS/XPLOR_distance_constraints_5	OK	3123 (1)	noe	87.4 (chain: A, length: 111)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	56 (1)	hbond	33.3 (chain: A, length: 111)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	136 (136)	.	66.7 (chain: A, length: 111)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_4	OK	60 (60)	.	54.1 (chain: A, length: 111)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 111, Sequence coverage: 87.4%
- Total number of assignments
  - ¹H chemical shifts: 607
  - ¹³C chemical shifts: 433
  - ¹⁵N chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	689	607	88.1
¹³C chemical shifts	498	433	86.9
¹⁵N chemical shifts	120	104	86.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	227	199	87.7
¹³C chemical shifts	222	190	85.6
¹⁵N chemical shifts	107	94	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	462	408	88.3
¹³C chemical shifts	276	243	88.0
¹⁵N chemical shifts	13	10	76.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	45	97.8
¹³C chemical shifts	46	45	97.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	45	30	66.7
¹³C chemical shifts	45	30	66.7

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_5
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 111, Sequence coverage: 87.4%
- Total number of restraints: 3117
- Weight of restraints: 1.0 (3117)
- Potential type of restraints: square-well-parabolic (3117)
- Range of distance target values: 2.09, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 111, Sequence coverage: 33.3%
  - Total number of restraints: 56
  - Weight of restraints: 1.0 (56)
  - Potential type of restraints: square-well-parabolic (56)
  - Range of distance target values: 1.96, 2.46 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 111, Sequence coverage: 66.7%
- Total number of restraints: 136
- Total number of restraints per polymer type: 136
- Weight of restraints: 1.0 (136)
- Potential type of restraints: square-well-parabolic (136)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_4

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 111, Sequence coverage: 54.1%

----------------------10--------20--------30--------40--------50--------60--------70--------80------
MGSSHHHHHHSQDPMSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEED
               | | |  ||  |||  ||| | ||||| | |||| |  ||  |  ||| || | |   ||||| ||| | | |||    ||| ||
...............S.Q.A..ST..LGD..EGE.I.LKVIG.D.SEIH.K..MT..L..LKE.YC.R.G...NSLRF.FEG.R.A.NHT....GME.ED
----------------------10--------20--------30--------40--------50--------60--------70--------80------

--90-------
VIEVYQEQTGG
||||| |||| 
VIEVY.EQTG 
--90------

Total number of restraints: 60
Potential type of restraints: undefined (60)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords SUMO, Ubiquitin-like protein

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Sequence search (BLAST)

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Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.033 seconds)

BMRB: 25576

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. human SUMO1, : 1 : 27 : 41 entities Detail

Interaction partners 1. human SUMO1, : 123 interactors Detail

Experiments performed 17 experiments Detail

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NMR combined restraints 6 contents Detail