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BMRB: 25587

2N26

Solution structures of Miz-1 zinc fingers 2 to 4

Authors

Bedard, M., Lavigne, P.

Assembly

Miz-1 zinc fingers 2 to 4

Entity

1. Miz-1 zinc fingers 2 to 4 (polymer, Thiol state: all free), 112 monomers, 12771.50 Da Detail

MVIHKCEDCG KEFTHTGNFK RHIRIHTGEK PFSCRECSKA FSDPAACKAH EKTHSPLKPY GCEECGKSYR LISLLNLHKK RHSGEARYRC EDCGKLFTTS GNLKRHQLVH SG

Formula weight

12771.5 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 91.1 %, Completeness: 73.8 %, Completeness (bb): 87.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.8 % (964 of 1307)	68.1 % (470 of 690)	78.2 % (395 of 505)	88.4 % (99 of 112)
Backbone	87.0 % (578 of 664)	85.2 % (195 of 229)	87.8 % (287 of 327)	88.9 % (96 of 108)
Sidechain	64.3 % (480 of 746)	59.7 % (275 of 461)	71.9 % (202 of 281)	75.0 % (3 of 4)
Aromatic	51.8 % (59 of 114)	52.6 % (30 of 57)	50.9 % (29 of 57)
Methyl	71.8 % (56 of 78)	69.2 % (27 of 39)	74.4 % (29 of 39)

1. Miz1-4

MVIHKCEDCG KEFTHTGNFK RHIRIHTGEK PFSCRECSKA FSDPAACKAH EKTHSPLKPY GCEECGKSYR LISLLNLHKK RHSGEARYRC EDCGKLFTTS GNLKRHQLVH SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.15 ppm, Outliers: 5 Detail

LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N26, Strand ID: A Detail

Release date

2016-03-21

Citation

A beta-sheet interaction interface directs the tetramerisation of the Miz-1 POZ domain
Bedard, M., Lavigne, P.
J. Mol. Biol. (2007), 373, 820-826, PubMed 17880999 , DOI 10.1016/j.jmb.2007.08.026 , Abstract

Related entities 1. Miz-1 zinc fingers 2 to 4, : 1 : 1 : 2 : 2240 entities Detail

Interaction partners 1. Miz-1 zinc fingers 2 to 4, : 21 interactors Detail

More details for 21 interactors identified by 12 citations

Experiments performed 11 experiments Detail

1 cbcaconh (H[N[co[{CA|ca[C]}]]])

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

2 3D HNCO

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

3 hnha (H{[N]+[HA]})

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

4 3D 1H-15N NOESY

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

5 2D 1H-15N HSQC/HMQC

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

6 3D HNCACB

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

7 ccconh (HNCO)

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

8 3D 1H-13C NOESY

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

9 3D HCCH-TOCSY

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

10 2D 1H-13C HSQC/HMQC

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

11 3D 1H-13C NOESY

Instrument

Varian UnityInova - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM acetic acid, 10 mM cacodylate, pH 6.5, 50 mM KCl, 2 mM TCEP, 5 eq. ZnCl2

#	Name	Isotope labeling	Type	Concentration
1	Miz1-4	[U-13C; U-15N]		0.7 ~ 1.0 mM
2	acetic acid	natural abundance		10 mM
3	cacodylate	natural abundance		10 mM
4	KCl	natural abundance		50 mM
5	TCEP	natural abundance		2 mM
6	ZnCl2	natural abundance		5 eq
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

nullKeywords C2H2 zinc finger, Miz-1, ZBTB17