BMRBj - BMREntryMaster

Found 1 Document (0.509 seconds)

BMRB: 25596

2N2D

Structure of DNA G-quadruplex adopted by ALS and FTD related GGGGCC repeat with G21 to Br-G21 substitution

Authors

Brcic, J., Plavec, J.

Assembly

G-quadruplex

Entity

1. G-quadruplex (polymer, Thiol state: not present), 22 monomers, 7099.293 Da Detail

GGGGCCGGGG CCGGGGCCGG XG

Formula weight

7099.293 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 67.2 %, Completeness (bb): 60.5 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	67.2 % (135 of 201)	67.2 % (135 of 201)
Suger, PO4	60.5 % (89 of 147)	60.5 % (89 of 147)
Nucleobase	85.2 % (46 of 54)	85.2 % (46 of 54)
Aromatic	100.0 % (42 of 42)	100.0 % (42 of 42)

1. GGGGCC repeat G-quadruplex

GGGGCCGGGG CCGGGGCCGG XG

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details 0.5 mM DNA, 120 mM KCl, 20 mM potassium phosphate buffer pH 7.2, 90% H2O, 10% D2O

#	Name	Isotope labeling	Concentration
1	dna	natural abundance	0.5 mM
2	potassium chloride	natural abundance	120 mM
3	potassium phosphate	natural abundance	20 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details 0.5 mM DNA, 120 mM KCl, 20 mM potassium phosphate buffer pH 7.2, 100% D2O

#	Name	Isotope labeling	Concentration
6	dna	natural abundance	0.5 mM
7	potassium chloride	natural abundance	120 mM
8	potassium phosphate	natural abundance	20 mM
9	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details 0.3-0.6 mM 8% 15, 8% 13C N DNA, 120 mM KCl, 20 mM potassium phosphate buffer pH 7.2, 90% H2O, 10% D2O

#	Name	Isotope labeling	Concentration
10	dna	8% 13C, 8% 15 N	0.3 ~ 0.6 mM
11	potassium chloride	natural abundance	120 mM
12	potassium phosphate	natural abundance	20 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details 0.3-0.6 mM 8% 15 N DNA, 120 mM KCl, 20 mM potassium phosphate buffer pH 7.2, 90% H2O, 10% D2O

#	Name	Isotope labeling	Concentration
15	dna	8% 15 N	0.3 ~ 0.6 mM
16	potassium chloride	natural abundance	120 mM
17	potassium phosphate	natural abundance	20 mM
18	H2O	natural abundance	90 %
19	D2O	natural abundance	10 %

Release date

2015-09-20

Citation

Solution structure of a DNA quadruplex containing ALS and FTD related GGGGCC repeat stabilized by 8-bromodeoxyguanosine substitution
Brcic, J., Plavec, J.
Nucleic Acids Res. (2015), 43, 8590-8600, PubMed 26253741 , DOI 10.1093/nar/gkv815 , Abstract