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BMRB: 25599

2N2H

Solution structure of Sds3 in complex with Sin3A

Authors

Clark, M., Radhakrishnan, I.

Assembly

Sds3 in complex with Sin3A

Entity

1. Sds3SID (polymer, Thiol state: not present), 27 monomers, 3122.505 Da Detail

SNAAQLNYLL TDEQIMEDLR TLNKLKS

2. Sin3HID (polymer), 125 monomers, 14672.61 Da Detail

SNAEHIYRCE DERFELDVVL ETNLATIRVL EAIQKKLSRL SAEEQAKFRL DNTLGGTSEV IHRKALQRIY ADKAADIIDG LRKNPSIAVP IVLKRLKMKE EEWREAQRGF NKVWREQNEK YYLKS

Total weight

17795.115 Da

Max. entity weight

14672.61 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 82.9 %, Completeness: 75.7 %, Completeness (bb): 81.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.7 % (1402 of 1853)	72.6 % (709 of 977)	79.4 % (562 of 708)	78.0 % (131 of 168)
Backbone	81.2 % (737 of 908)	80.7 % (247 of 306)	82.3 % (372 of 452)	78.7 % (118 of 150)
Sidechain	72.0 % (787 of 1093)	68.9 % (462 of 671)	77.2 % (312 of 404)	72.2 % (13 of 18)
Aromatic	57.8 % (59 of 102)	60.8 % (31 of 51)	53.1 % (26 of 49)	100.0 % (2 of 2)
Methyl	80.2 % (146 of 182)	80.2 % (73 of 91)	80.2 % (73 of 91)

1. Sds3SID

SNAAQLNYLL TDEQIMEDLR TLNKLKS

2. Sin3HID

SNAEHIYRCE DERFELDVVL ETNLATIRVL EAIQKKLSRL SAEEQAKFRL DNTLGGTSEV IHRKALQRIY ADKAADIIDG LRKNPSIAVP IVLKRLKMKE EEWREAQRGF NKVWREQNEK YYLKS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
1	Sin3HID	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	Sds3SID	natural abundance	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
5	Sds3SID	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
6	Sin3HID	natural abundance	0.5 ~ 1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 39.5 %, Completeness: 51.2 %, Completeness (bb): 55.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.2 % (1899 of 3706)	50.1 % (979 of 1954)	52.0 % (737 of 1416)	54.5 % (183 of 336)
Backbone	55.4 % (1006 of 1816)	55.9 % (342 of 612)	55.0 % (497 of 904)	55.7 % (167 of 300)
Sidechain	48.8 % (1067 of 2186)	47.5 % (637 of 1342)	51.2 % (414 of 808)	44.4 % (16 of 36)
Aromatic	29.9 % (61 of 204)	32.4 % (33 of 102)	26.5 % (26 of 98)	50.0 % (2 of 4)
Methyl	54.7 % (199 of 364)	54.4 % (99 of 182)	54.9 % (100 of 182)

1. Sds3SID

SNAAQLNYLL TDEQIMEDLR TLNKLKS

2. Sin3HID

SNAEHIYRCE DERFELDVVL ETNLATIRVL EAIQKKLSRL SAEEQAKFRL DNTLGGTSEV IHRKALQRIY ADKAADIIDG LRKNPSIAVP IVLKRLKMKE EEWREAQRGF NKVWREQNEK YYLKS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
1	Sin3HID	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	Sds3SID	natural abundance	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
5	Sds3SID	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
6	Sin3HID	natural abundance	0.5 ~ 1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2N2H, Strand ID: A, B Detail

Release date

2015-07-12

Citation

Structural insights into the assembly of the histone deacetylase-associated Sin3L/Rpd3L corepressor complex
Clark, M., Marcum, R., Graveline, R., Chan, C.W., Xie, T., Chen, Z., Ding, Y., Zhang, Y., Mondragon, A., David, G., Radhakrishnan, I.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, E3669-E3678, PubMed 26124119 , DOI 10.1073/pnas.1504021112 , Abstract

Related entities 1. Sds3SID, : 7 : 1 entities Detail

Related entities 2. Sin3HID, : 5 : 47 entities Detail

Interaction partners 1. Sds3SID, : 17 : 1 interactors Detail

More details for 18 interactors identified by 10 citations

Interaction partners 2. Sin3HID, : 54 interactors Detail

More details for 54 interactors identified by 29 citations

Experiments performed 8 experiments Detail

1 3D 1H-15N NOESY