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Found 1 Document (7.369 seconds)

BMRB: 25639

2N3A

Solution structure of LEDGF/p75 IBD in complex with POGZ peptide (1389-1404)

Authors

Tesina, P., Cermakova, K., Horejsi, M., Prochazkova, K., Fabry, M., Sharma, S., Christ, F., Demeulemeester, J., Debyser, Z., De Rijck, J., Veverka, V., Rezacova, P.

Assembly

LEDGF/p75 IBD in complex with POGZ peptide (1389-1404)

Entity

1. LEDGF/p75 IBD in complex with POGZ peptide (1389-1404), entity 1 (polymer, Thiol state: not present), 16 monomers, 1825.747 Da Detail

EGESETESFY GFEEAD

2. LEDGF/p75 IBD in complex with POGZ peptide (1389-1404), entity 2 (polymer, Thiol state: all free), 79 monomers, 9338.918 Da Detail

MDSRLQRIHA EIKNSLKIDN LDVNRCIEAL DELASLQVTM QQAQKHTEMI TTLKKIRRFK VSQVIMEKST MLYNKFKNM

Total weight

11164.665 Da

Max. entity weight

9338.918 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 79.9 %, Completeness (bb): 82.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.9 % (921 of 1153)	81.5 % (495 of 607)	78.4 % (345 of 440)	76.4 % (81 of 106)
Backbone	82.1 % (468 of 570)	84.4 % (162 of 192)	81.6 % (231 of 283)	78.9 % (75 of 95)
Sidechain	78.7 % (532 of 676)	80.2 % (333 of 415)	77.2 % (193 of 250)	54.5 % (6 of 11)
Aromatic	31.3 % (20 of 64)	56.3 % (18 of 32)	6.3 % (2 of 32)
Methyl	96.9 % (95 of 98)	98.0 % (48 of 49)	95.9 % (47 of 49)

1. entity 1

EGESETESFY GFEEAD

2. entity 2

MDSRLQRIHA EIKNSLKIDN LDVNRCIEAL DELASLQVTM QQAQKHTEMI TTLKKIRRFK VSQVIMEKST MLYNKFKNM

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	0.5 mM
2	entity_2	[U-13C; U-15N]	0.5 mM
3	HEPES	natural abundance	25 mM
4	NaCl	natural abundance	100 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 29 models in PDB: 2N3A, Strand ID: A, B Detail

Release date

2015-08-16

Citation

Multiple cellular proteins interact with LEDGF/p75 through a conserved unstructured consensus motif
Tesina, P., Cermakova, K., Horejsi, M., Prochazkova, K., Fabry, M., Sharma, S., Christ, F., Demeulemeester, J., Debyser, Z., Rijck, J., Veverka, V., Rezacova, P.
Nat. Commun. (2015), 6, 7968-7968, PubMed 26245978 , DOI 10.1038/ncomms8968 , Abstract

Related entities 1. LEDGF/p75 IBD in complex with POGZ peptide (1389-1404), entity 1, : 5 entities Detail

Related entities 2. LEDGF/p75 IBD in complex with POGZ peptide (1389-1404), entity 2, : 7 : 1 : 16 entities Detail

Interaction partners 1. LEDGF/p75 IBD in complex with POGZ peptide (1389-1404), entity 1, : 87 interactors Detail

More details for 87 interactors identified by 15 citations

Interaction partners 2. LEDGF/p75 IBD in complex with POGZ peptide (1389-1404), entity 2, : 13 interactors Detail

More details for 13 interactors identified by 3 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC