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BMRB: 25652

2N3O

Structure of PTB RRM1(41-163) bound to an RNA stemloop containing a structured loop derived from viral internal ribosomal entry site RNA

Authors

Maris, C., Jayne, S.F., Damberger, F.F., Ravindranathan, S., Allain, F.H.T.

Assembly

PTBRRM1-SL23H complex

Entity

1. PTBRRM1 (polymer, Thiol state: not present), 123 monomers, 13359.03 Da Detail

GNDSKKFKGD SRSAGVPSRV IHIRKLPIDV TEGEVISLGL PFGKVTNLLM LKGKNQAFIE MNTEEAANTM VNYYTSVTPV LRGQPIYIQF SNHKELKTDS SPNQARAQAA LQAVNSVQSG NLA

2. RNA (5'-R(*GP*GP*GP*AP*CP*CP*UP*GP*GP*UP*CP*UP*UP*UP*CP*CP*AP*GP*GP*UP*CP*CP*C)-3') (polymer, Thiol state: not present), 23 monomers, 7371.315 Da Detail

GGGACCUGGU CUUUCCAGGU CCC

Total weight

20730.346 Da

Max. entity weight

13359.03 Da

Source organism

Homo sapiens , Encephalomyocarditis

Exptl. method

solution NMR

Refine. method

simulated annealing, simulated annealing

Data set

RDCs, assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 83.6 %, Completeness: 69.0 %, Completeness (bb): 61.2 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	69.0 % (1231 of 1785)	74.3 % (700 of 942)	58.9 % (410 of 696)	82.3 % (121 of 147)
Backbone	61.2 % (599 of 979)	62.8 % (243 of 387)	51.4 % (244 of 475)	95.7 % (112 of 117)
Sidechain	80.7 % (742 of 920)	82.3 % (457 of 555)	82.4 % (276 of 335)	30.0 % (9 of 30)
Aromatic	33.0 % (58 of 176)	39.8 % (35 of 88)	30.7 % (23 of 75)	0.0 % (0 of 13)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. PTBRRM1

GNDSKKFKGD SRSAGVPSRV IHIRKLPIDV TEGEVISLGL PFGKVTNLLM LKGKNQAFIE MNTEEAANTM VNYYTSVTPV LRGQPIYIQF SNHKELKTDS SPNQARAQAA LQAVNSVQSG NLA

2. RNA (5'-R(*GP*GP*GP*AP*CP*CP*UP*GP*GP*UP*CP*UP*UP*UP*CP*CP*AP*GP*GP*UP*CP*CP*C)-3')

GGGACCUGGU CUUUCCAGGU CCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
2	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
3	sodium phosphate	natural abundance	10 (±0.1) mM
4	sodium chloride	natural abundance	20 (±0.2) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
18	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
19	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Ura10,Ura12,Ura14	1 (±0.2) mM
20	sodium phosphate	natural abundance	10 (±0.1) mM
21	sodium chloride	natural abundance	20 (±0.2) mM
22	D2O	natural abundance	100 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
23	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
24	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
25	sodium phosphate	natural abundance	10 (±0.1) mM
26	sodium chloride	natural abundance	20 (±0.2) mM
27	D2O	natural abundance	100 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
28	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
29	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
30	sodium phosphate	natural abundance	10 (±0.1) mM
31	sodium chloride	natural abundance	20 (±0.2) mM
32	D2O	natural abundance	100 %

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Pf1 phage added for orienting macromolecules to obtain RCS. Compared to sample without Pf1 phage.

#	Name	Isotope labeling	Concentration
33	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
34	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
35	sodium phosphate	natural abundance	10 (±0.1) mM
36	sodium chloride	natural abundance	20 (±0.2) mM
37	Pf1 phage	natural abundance	13 mg/mL
38	H2O	natural abundance	90 %
39	D2O	natural abundance	10 %

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Pf1 phage added for orienting macromolecules to obtain RCS. Compared to sample without Pf1 phage.

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

Sample #9

#	Name	Isotope labeling	Concentration
46	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
47	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
48	sodium phosphate	natural abundance	10 (±0.1) mM
49	sodium chloride	natural abundance	20 (±0.2) mM
50	Pf1 phage	natural abundance	13 mg/mL
51	H2O	natural abundance	90 %
52	D2O	natural abundance	10 %

Sample #10

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
53	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
54	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
55	sodium phosphate	natural abundance	10 (±0.1) mM
56	sodium chloride	natural abundance	20 (±0.2) mM
57	H2O	natural abundance	90 %
58	D2O	natural abundance	10 %

Sample #11

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details ribose at positions 9,11,13 and 15 are 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
59	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
60	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Cyt9,Ura11,Ura13,Gua15	1 (±0.2) mM
61	sodium phosphate	natural abundance	10 (±0.1) mM
62	sodium chloride	natural abundance	20 (±0.2) mM
63	D2O	natural abundance	100 %

Sample #12

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Only cytosine and uracil nucleotides are uniformly 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
64	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
65	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-Ura7,Ura10,Cyt11,Ura12,Ura13,Ura14,Cyt15,Cyt16,Ura20,Cyt21,Cyt22,Cyt23	1 (±0.2) mM
66	sodium phosphate	natural abundance	10 (±0.1) mM
67	sodium chloride	natural abundance	20 (±0.2) mM
68	D2O	natural abundance	100 %

Sample #13

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
69	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
70	sodium phosphate	natural abundance	10 (±0.1) mM
71	sodium chloride	natural abundance	20 (±0.2) mM
72	D2O	natural abundance	100 %

Sample #14

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
73	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
74	sodium phosphate	natural abundance	10 (±0.1) mM
75	sodium chloride	natural abundance	20 (±0.2) mM
76	H2O	natural abundance	90 %
77	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 45.2 %, Completeness: 54.0 %, Completeness (bb): 54.0 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	54.0 % (1928 of 3570)	57.6 % (1086 of 1884)	49.0 % (682 of 1392)	54.4 % (160 of 294)
Backbone	54.0 % (1057 of 1958)	59.6 % (461 of 774)	47.3 % (449 of 950)	62.8 % (147 of 234)
Sidechain	55.0 % (1012 of 1840)	56.4 % (626 of 1110)	55.7 % (373 of 670)	21.7 % (13 of 60)
Aromatic	41.5 % (146 of 352)	46.0 % (81 of 176)	43.3 % (65 of 150)	0.0 % (0 of 26)
Methyl	60.2 % (171 of 284)	62.0 % (88 of 142)	58.5 % (83 of 142)

1. PTBRRM1

GNDSKKFKGD SRSAGVPSRV IHIRKLPIDV TEGEVISLGL PFGKVTNLLM LKGKNQAFIE MNTEEAANTM VNYYTSVTPV LRGQPIYIQF SNHKELKTDS SPNQARAQAA LQAVNSVQSG NLA

2. RNA (5'-R(*GP*GP*GP*AP*CP*CP*UP*GP*GP*UP*CP*UP*UP*UP*CP*CP*AP*GP*GP*UP*CP*CP*C)-3')

GGGACCUGGU CUUUCCAGGU CCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
2	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
3	sodium phosphate	natural abundance	10 (±0.1) mM
4	sodium chloride	natural abundance	20 (±0.2) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
18	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
19	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Ura10,Ura12,Ura14	1 (±0.2) mM
20	sodium phosphate	natural abundance	10 (±0.1) mM
21	sodium chloride	natural abundance	20 (±0.2) mM
22	D2O	natural abundance	100 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
23	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
24	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
25	sodium phosphate	natural abundance	10 (±0.1) mM
26	sodium chloride	natural abundance	20 (±0.2) mM
27	D2O	natural abundance	100 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
28	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
29	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
30	sodium phosphate	natural abundance	10 (±0.1) mM
31	sodium chloride	natural abundance	20 (±0.2) mM
32	D2O	natural abundance	100 %

Sample #7

#	Name	Isotope labeling	Concentration
33	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
34	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
35	sodium phosphate	natural abundance	10 (±0.1) mM
36	sodium chloride	natural abundance	20 (±0.2) mM
37	Pf1 phage	natural abundance	13 mg/mL
38	H2O	natural abundance	90 %
39	D2O	natural abundance	10 %

Sample #8

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

Sample #9

#	Name	Isotope labeling	Concentration
46	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
47	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
48	sodium phosphate	natural abundance	10 (±0.1) mM
49	sodium chloride	natural abundance	20 (±0.2) mM
50	Pf1 phage	natural abundance	13 mg/mL
51	H2O	natural abundance	90 %
52	D2O	natural abundance	10 %

Sample #10

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
53	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
54	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
55	sodium phosphate	natural abundance	10 (±0.1) mM
56	sodium chloride	natural abundance	20 (±0.2) mM
57	H2O	natural abundance	90 %
58	D2O	natural abundance	10 %

Sample #11

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details ribose at positions 9,11,13 and 15 are 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
59	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
60	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Cyt9,Ura11,Ura13,Gua15	1 (±0.2) mM
61	sodium phosphate	natural abundance	10 (±0.1) mM
62	sodium chloride	natural abundance	20 (±0.2) mM
63	D2O	natural abundance	100 %

Sample #12

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Only cytosine and uracil nucleotides are uniformly 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
64	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
65	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-Ura7,Ura10,Cyt11,Ura12,Ura13,Ura14,Cyt15,Cyt16,Ura20,Cyt21,Cyt22,Cyt23	1 (±0.2) mM
66	sodium phosphate	natural abundance	10 (±0.1) mM
67	sodium chloride	natural abundance	20 (±0.2) mM
68	D2O	natural abundance	100 %

Sample #13

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
69	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
70	sodium phosphate	natural abundance	10 (±0.1) mM
71	sodium chloride	natural abundance	20 (±0.2) mM
72	D2O	natural abundance	100 %

Sample #14

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
73	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
74	sodium phosphate	natural abundance	10 (±0.1) mM
75	sodium chloride	natural abundance	20 (±0.2) mM
76	H2O	natural abundance	90 %
77	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N3O, Strand ID: A Detail

RDC

70 RDC values in 1 lists
Field strength (¹H) 900.0 MHz, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1) Detail

Release date

2016-08-07

Related entities 1. PTBRRM1, : 1 : 3 : 49 entities Detail

Experiments performed 43 experiments Detail

1 3D HNCA

Instrument

Bruker Avance - 700 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

2 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

3 3D HNCACB

Instrument

Bruker Avance - 500 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

5 3D H(C)CH-TOCSY

Instrument

Bruker Avance - 700 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

6 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

7 3D 1H-15N NOESY-HSQC

Instrument

Bruker Avance - 700 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
2	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
3	sodium phosphate	natural abundance	10 (±0.1) mM
4	sodium chloride	natural abundance	20 (±0.2) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 3D 1H-13C NOESY aliphatic-HSQC

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

9 3D 1H-13C NOESY aromatic-HSQC

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

10 2D 1H-1H TOCSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
23	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
24	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
25	sodium phosphate	natural abundance	10 (±0.1) mM
26	sodium chloride	natural abundance	20 (±0.2) mM
27	D2O	natural abundance	100 %

11 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
23	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
24	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
25	sodium phosphate	natural abundance	10 (±0.1) mM
26	sodium chloride	natural abundance	20 (±0.2) mM
27	D2O	natural abundance	100 %

12 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC

Instrument

Bruker Avance - 700 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

13 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
18	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
19	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Ura10,Ura12,Ura14	1 (±0.2) mM
20	sodium phosphate	natural abundance	10 (±0.1) mM
21	sodium chloride	natural abundance	20 (±0.2) mM
22	D2O	natural abundance	100 %

14 2D f2-13C-filtered NOESY

Instrument

Bruker Avance - 700 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

15 3D aliphatic-HSQC-NOESY

Instrument

Bruker Avance - 500 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	PTBRRM1	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
8	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
9	sodium phosphate	natural abundance	10 (±0.1) mM
10	sodium chloride	natural abundance	20 (±0.2) mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

16 2D H2C2 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

17 2D H6C6 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

18 2D H8C8 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

19 2D H2C2 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Pf1 phage added for orienting macromolecules to obtain RCS. Compared to sample without Pf1 phage.

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

20 2D H6C6 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

21 2D H8C8 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

22 2D H5C5 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

23 2D H1'C1' TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

24 2D H5C5 TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

25 2D H1'C1' TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

#	Name	Isotope labeling	Concentration
40	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
41	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
42	sodium phosphate	natural abundance	10 (±0.1) mM
43	sodium chloride	natural abundance	20 (±0.2) mM
44	Pf1 phage	natural abundance	13 mg/mL
45	D2O	natural abundance	100 %

26 2D HN TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
2	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
3	sodium phosphate	natural abundance	10 (±0.1) mM
4	sodium chloride	natural abundance	20 (±0.2) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

27 2D HN TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Pf1 phage added for orienting macromolecules to obtain RCS. Compared to sample without Pf1 phage.

#	Name	Isotope labeling	Concentration
33	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
34	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
35	sodium phosphate	natural abundance	10 (±0.1) mM
36	sodium chloride	natural abundance	20 (±0.2) mM
37	Pf1 phage	natural abundance	13 mg/mL
38	H2O	natural abundance	90 %
39	D2O	natural abundance	10 %

28 2D HN TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
53	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
54	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
55	sodium phosphate	natural abundance	10 (±0.1) mM
56	sodium chloride	natural abundance	20 (±0.2) mM
57	H2O	natural abundance	90 %
58	D2O	natural abundance	10 %

29 2D HN TROSY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 (±1) K, pH 6.5 (±0.1), Details Pf1 phage added for orienting macromolecules to obtain RCS. Compared to sample without Pf1 phage.

#	Name	Isotope labeling	Concentration
46	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
47	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
48	sodium phosphate	natural abundance	10 (±0.1) mM
49	sodium chloride	natural abundance	20 (±0.2) mM
50	Pf1 phage	natural abundance	13 mg/mL
51	H2O	natural abundance	90 %
52	D2O	natural abundance	10 %

30 2D {1H}15N-NOE HSQC

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
2	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
3	sodium phosphate	natural abundance	10 (±0.1) mM
4	sodium chloride	natural abundance	20 (±0.2) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

31 2D 1H-1H NOESY

Instrument

Bruker Avance - 750 MHz RT probehead

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
2	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
3	sodium phosphate	natural abundance	10 (±0.1) mM
4	sodium chloride	natural abundance	20 (±0.2) mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

32 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC

Instrument

Bruker Avance - 700 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Only cytosine and uracil nucleotides are uniformly 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
64	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
65	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-Ura7,Ura10,Cyt11,Ura12,Ura13,Ura14,Cyt15,Cyt16,Ura20,Cyt21,Cyt22,Cyt23	1 (±0.2) mM
66	sodium phosphate	natural abundance	10 (±0.1) mM
67	sodium chloride	natural abundance	20 (±0.2) mM
68	D2O	natural abundance	100 %

33 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 750 MHz RT probehead

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details Only cytosine and uracil nucleotides are uniformly 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
64	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
65	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-Ura7,Ura10,Cyt11,Ura12,Ura13,Ura14,Cyt15,Cyt16,Ura20,Cyt21,Cyt22,Cyt23	1 (±0.2) mM
66	sodium phosphate	natural abundance	10 (±0.1) mM
67	sodium chloride	natural abundance	20 (±0.2) mM
68	D2O	natural abundance	100 %

34 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
18	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
19	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Ura10,Ura12,Ura14	1 (±0.2) mM
20	sodium phosphate	natural abundance	10 (±0.1) mM
21	sodium chloride	natural abundance	20 (±0.2) mM
22	D2O	natural abundance	100 %

35 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details ribose at positions 9,11,13 and 15 are 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
59	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
60	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Cyt9,Ura11,Ura13,Gua15	1 (±0.2) mM
61	sodium phosphate	natural abundance	10 (±0.1) mM
62	sodium chloride	natural abundance	20 (±0.2) mM
63	D2O	natural abundance	100 %

36 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
18	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
19	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Ura10,Ura12,Ura14	1 (±0.2) mM
20	sodium phosphate	natural abundance	10 (±0.1) mM
21	sodium chloride	natural abundance	20 (±0.2) mM
22	D2O	natural abundance	100 %

37 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

38 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
14	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
15	sodium phosphate	natural abundance	10 (±0.1) mM
16	sodium chloride	natural abundance	20 (±0.2) mM
17	D2O	natural abundance	100 %

39 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
23	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
24	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
25	sodium phosphate	natural abundance	10 (±0.1) mM
26	sodium chloride	natural abundance	20 (±0.2) mM
27	D2O	natural abundance	100 %

40 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
69	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
70	sodium phosphate	natural abundance	10 (±0.1) mM
71	sodium chloride	natural abundance	20 (±0.2) mM
72	D2O	natural abundance	100 %

41 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
69	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-99% 13C; U-99% 15N]	1 (±0.2) mM
70	sodium phosphate	natural abundance	10 (±0.1) mM
71	sodium chloride	natural abundance	20 (±0.2) mM
72	D2O	natural abundance	100 %

42 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 600 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±1) K, pH 6.5 (±0.1), Details ribose at positions 9,11,13 and 15 are 13C,15N-labeled.

#	Name	Isotope labeling	Concentration
59	PTBRRM1	[U-99% 15N]	1 (±0.2) mM
60	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	[U-13C; U-15N]-ribose-Cyt9,Ura11,Ura13,Gua15	1 (±0.2) mM
61	sodium phosphate	natural abundance	10 (±0.1) mM
62	sodium chloride	natural abundance	20 (±0.2) mM
63	D2O	natural abundance	100 %

43 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz cryoprobe-equipped

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
73	RNA (5'-R(GPGPGPAPCPCPUPGPGPUPCPUPUPUPCPCPAPGPGPUPCPCP*C)-3')	natural abundance	1 (±0.2) mM
74	sodium phosphate	natural abundance	10 (±0.1) mM
75	sodium chloride	natural abundance	20 (±0.2) mM
76	H2O	natural abundance	90 %
77	D2O	natural abundance	10 %

NMR combined restraints 9 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25652_2n3o.nef
Input source #2: Coordindates	2n3o.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------50--------60--------70--------80--------90-------100-------110-------120-------130-------140
GNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDS
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDS
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-------150-------160---
SPNQARAQAALQAVNSVQSGNLA
|||||||||||||||||||||||
SPNQARAQAALQAVNSVQSGNLA
-------110-------120---

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20---
GGGACCUGGUCUUUCCAGGUCCC
|||||||||||||||||||||||
GGGACCUGGUCUUUCCAGGUCCC

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	123	0	0	100.0
B	B	23	0	0	100.0

Content subtype: combined_25652_2n3o.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	protein-chemical-shifts	Warning	1196		98.4 (chain: A, length: 123)
2	Assigned chemical shifts	RNA-chemical-shifts	OK	327		100.0 (chain: B, length: 23)
1	Distance restraints	DYANA/DIANA_distance_constraints_8	OK	2687 (1)	noe	93.5 (chain: A, length: 123)
2	Distance restraints	DYANA/DIANA_distance_constraints_10	OK	93 (1)	noe	39.1 (chain: B, length: 23)
3	Distance restraints	DYANA/DIANA_distance_constraints_12	Warning	131 (1)	noe	13.8 (chain: A, length: 123), 26.1 (chain: B, length: 23)
4	Distance restraints	DYANA/DIANA_distance_constraints_11	OK	379 (1)	undefined	100.0 (chain: B, length: 23)
5	Distance restraints	DYANA/DIANA_distance_constraints_7	Warning	110 (1)	hbond	32.5 (chain: A, length: 123), 91.3 (chain: B, length: 23)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_9	OK	383 (383)	.	100.0 (chain: B, length: 23)

Assigned chemical shifts

Saveframe: protein-chemical-shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 98.4%
- Total number of assignments
  - ¹H chemical shifts: 694
  - ¹³C chemical shifts: 383
  - ¹⁵N chemical shifts: 119
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: RNA-chemical-shifts
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 175
  - ¹³C chemical shifts: 152
- Completeness of assignments
  - Entity_assemble_ID: B
- Histogram of normalized assigned chemical shifts

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_8
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 93.5%
- Total number of restraints: 2687
- Weight of restraints: 1.0 (2687)
- Potential type of restraints: upper-bound-parabolic (2687)
- Range of distance target values: 2.4, 5.77 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
    None
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_10
  - Status: OK
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 39.1%
  - Total number of restraints: 93
  - Weight of restraints: 1.0 (93)
  - Potential type of restraints: upper-bound-parabolic (93)
  - Range of distance target values: 2.8, 6.0 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: A
      None
    - Chain_ID: B
  - Distance restraints on contact map
  - Saveframe: DYANA/DIANA_distance_constraints_12
    - Status: Warning
    - Experiment type: noe
    - Sequence coverage of experimental data
      - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 13.8%
      - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 26.1%
    - Total number of restraints: 130
    - Weight of restraints: 1.0 (130)
    - Potential type of restraints: upper-bound-parabolic (130)
    - Range of distance target values: 4.0, 6.0 (Å)
    - Histogram of distance target values
    - Histogram of discrepancy in distance target values
    - Distance restraints per residue
      - Chain_ID: A
      - Chain_ID: B
    - Distance restraints on contact map
    - Saveframe: DYANA/DIANA_distance_constraints_11
      - Status: OK
      - Experiment type: undefined
      - Sequence coverage of experimental data
        Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
        --------10--------20--- GGGACCUGGUCUUUCCAGGUCCC ||||||||||||||||||||||| GGGACCUGGUCUUUCCAGGUCCC
      - Total number of restraints: 379
        Intra-residue restraints, i = j : 195
        Sequential restraints, | i - j | = 1 : 119
        Backbone-backbone: 11
        Backbone-side chain: 86
        Side chain-side chain: 22
        Long range restraints, | i - j | >= 5 : 14
        Total hydrogen bond restraints: 51
        O...H-x: 2
        O...h-N: 2
        Long range: 47
        O...H-x: 16
        O...h-N: 14
        N...H-x: 9
        N...h_N: 8
      - Weight of restraints: 1.0 (379)
        Intra-residue restraints, i = j : 1.0 (195)
        Sequential restraints, | i - j | = 1 : 1.0 (119)
        Backbone-backbone: 1.0 (11)
        Backbone-side chain: 1.0 (86)
        Side chain-side chain: 1.0 (22)
        Long range restraints, | i - j | >= 5 : 1.0 (14)
        Total hydrogen bond restraints: 1.0 (51)
        O...H-x: 1.0 (2)
        O...h-N: 1.0 (2)
        Long range: 1.0 (47)
        O...H-x: 1.0 (16)
        O...h-N: 1.0 (14)
        N...H-x: 1.0 (9)
        N...h_N: 1.0 (8)
      - Potential type of restraints: upper-bound-parabolic (379)
        Intra-residue restraints, i = j : upper-bound-parabolic (195)
        Sequential restraints, | i - j | = 1 : upper-bound-parabolic (119)
        Backbone-backbone: upper-bound-parabolic (11)
        Backbone-side chain: upper-bound-parabolic (86)
        Side chain-side chain: upper-bound-parabolic (22)
        Long range restraints, | i - j | >= 5 : upper-bound-parabolic (14)
        Total hydrogen bond restraints: upper-bound-parabolic (51)
        O...H-x: upper-bound-parabolic (2)
        O...h-N: upper-bound-parabolic (2)
        Long range: upper-bound-parabolic (47)
        O...H-x: upper-bound-parabolic (16)
        O...h-N: upper-bound-parabolic (14)
        N...H-x: upper-bound-parabolic (9)
        N...h_N: upper-bound-parabolic (8)
      - Range of distance target values: 1.44, 18.0 (Å)
      - Histogram of distance target values
      - Histogram of discrepancy in distance target values
      - Distance restraints per residue
        Chain_ID: A
        None
        Chain_ID: B
      - Distance restraints on contact map
        Chain_ID_1: B - Chain_ID_2: B
      - Saveframe: DYANA/DIANA_distance_constraints_7
        Status: Warning
        Experiment type: hbond
        Sequence coverage of experimental data
        Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 32.5%
        Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 91.3%
        --------10--------20--- GGGACCUGGUCUUUCCAGGUCCC |||||||||| | |||||||||| GGGACCUGGU.U.UCCAGGUCCC
        Total number of restraints: 110
        Total hydrogen bond restraints: 110
        O...H-x (too close!): 19
        O...H-x: 19
        Long range: 68
        O...H-x (too close!): 25
        N...H-x (too close!): 9
        O...H-x: 25
        N...H-x: 9
        Inter-chain: 4
        O...H-x: 2
        O...h-N: 2
        Weight of restraints: 1.0 (110)
        Total hydrogen bond restraints: 1.0 (110)
        O...H-x (too close!): 1.0 (19)
        O...H-x: 1.0 (19)
        Long range: 1.0 (68)
        O...H-x (too close!): 1.0 (25)
        N...H-x (too close!): 1.0 (9)
        O...H-x: 1.0 (25)
        N...H-x: 1.0 (9)
        Inter-chain: 1.0 (4)
        O...H-x: 1.0 (2)
        O...h-N: 1.0 (2)
        Potential type of restraints: upper-bound-parabolic (110)
        Total hydrogen bond restraints: upper-bound-parabolic (110)
        O...H-x (too close!): upper-bound-parabolic (19)
        O...H-x: upper-bound-parabolic (19)
        Long range: upper-bound-parabolic (68)
        O...H-x (too close!): upper-bound-parabolic (25)
        N...H-x (too close!): upper-bound-parabolic (9)
        O...H-x: upper-bound-parabolic (25)
        N...H-x: upper-bound-parabolic (9)
        Inter-chain: upper-bound-parabolic (4)
        O...H-x: upper-bound-parabolic (2)
        O...h-N: upper-bound-parabolic (2)
        Range of distance target values: 1.8, 3.4 (Å)
        Histogram of distance target values
        Histogram of discrepancy in distance target values
        Distance restraints per residue
        Chain_ID: A
        Chain_ID: B
        Distance restraints on contact map
        Chain_ID_1: B - Chain_ID_2: B

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_9
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
- Total number of restraints: 383
- Total number of restraints per polymer type: 383
- Weight of restraints: 1.0 (383)
- Potential type of restraints: square-well-parabolic (383)
- Dihedral angle restraints per residue
  - Chain_ID: B

Keywords C-terminal helix formation, Encephalomyocarditis virus, IRES, PTB, Polypyirimine tract binding protein, Protein/RNA

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	736	694	94.3
¹³C chemical shifts	542	383	70.7
¹⁵N chemical shifts	139	119	85.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	249	242	97.2
¹³C chemical shifts	246	112	45.5
¹⁵N chemical shifts	117	109	93.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	487	452	92.8
¹³C chemical shifts	296	271	91.6
¹⁵N chemical shifts	22	10	45.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	206	175	85.0
¹³C chemical shifts	154	152	98.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	138	137	99.3
¹³C chemical shifts	115	114	99.1

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Found 1 Document (0.885 seconds)

BMRB: 25652

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PTBRRM1, : 1 : 3 : 49 entities Detail

Experiments performed 43 experiments Detail

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