NMR structure of the II-III-VI three-way junction from the VS ribozyme
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.4 % (921 of 986) | 92.4 % (502 of 543) | 95.2 % (394 of 414) | 86.2 % (25 of 29) |
Suger, PO4 | 91.9 % (627 of 682) | 90.6 % (337 of 372) | 93.5 % (290 of 310) | |
Nucleobase | 96.7 % (294 of 304) | 96.5 % (165 of 171) | 100.0 % (104 of 104) | 86.2 % (25 of 29) |
Aromatic | 97.0 % (258 of 266) | 97.0 % (129 of 133) | 100.0 % (104 of 104) | 86.2 % (25 of 29) |
1. RNA (62-MER)
GCAGCAGGGA ACUCACGCUU GCGUAGAGGC UAAGUGCUUC GGCACAGCAC AAGCCCGCUG CGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (62-MER) | [U-15N] | 2 mM | |
2 | D2O | natural abundance | 10 % | |
3 | H2O | natural abundance | 90 % | |
4 | NaCacodylate | natural abundance | 10 mM | |
5 | KCl | natural abundance | 50 mM | |
6 | NaN3 | natural abundance | 0.05 mM | |
7 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | RNA (62-MER) | [U-13C; U-15N] | 2 mM | |
9 | D2O | natural abundance | 100 % | |
10 | NaCacodylate | natural abundance | 10 mM | |
11 | KCl | natural abundance | 50 mM | |
12 | NaN3 | natural abundance | 0.05 mM | |
13 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | RNA (62-MER) | [U-13C; U-15N]-Ade | 2 mM | |
15 | D2O | natural abundance | 100 % | |
16 | NaCacodylate | natural abundance | 10 mM | |
17 | KCl | natural abundance | 50 mM | |
18 | NaN3 | natural abundance | 0.05 mM | |
19 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | RNA (62-MER) | [U-13C; U-15N]-Cyt | 2 mM | |
21 | D2O | natural abundance | 100 % | |
22 | NaCacodylate | natural abundance | 10 mM | |
23 | KCl | natural abundance | 50 mM | |
24 | NaN3 | natural abundance | 0.05 mM | |
25 | MgCl2 | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
26 | RNA (62-MER) | [U-13C; U-15N]-Gua | 2.5 mM | |
27 | D2O | natural abundance | 100 % | |
28 | NaCacodylate | natural abundance | 10 mM | |
29 | KCl | natural abundance | 50 mM | |
30 | NaN3 | natural abundance | 0.05 mM | |
31 | MgCl2 | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
32 | RNA (62-MER) | [U-13C; U-15N] | 2 mM | |
33 | D2O | natural abundance | 10 % | |
34 | H2O | natural abundance | 90 % | |
35 | NaCacodylate | natural abundance | 10 mM | |
36 | KCl | natural abundance | 50 mM | |
37 | NaN3 | natural abundance | 0.05 mM | |
38 | MgCl2 | natural abundance | 5 mM |