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BMRB: 25656

2N3S

NMR Assignments and structure of Translation initiation factor IF-1 from Burkholderia thailandensis E264.

Authors

Barnwal, R., Varani, G.

Assembly

Translation initiation factor IF-1 from Burkholderia thailandensis E264

Entity

1. Translation initiation factor IF-1 from Burkholderia thailandensis E264 (polymer, Thiol state: not present), 72 monomers, 8217.573 Da Detail

MAKDDVIQMQ GEVIENLPNA TFRVKLENGH VVLGHISGKM RMHYIRILPG DKVTVELTPY DLSRARIVFR AK

Formula weight

8217.573 Da

Source organism

Burkholderia thailandensis

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 76.7 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.7 % (660 of 861)	69.6 % (313 of 450)	81.9 % (276 of 337)	95.9 % (71 of 74)
Backbone	95.5 % (407 of 426)	93.8 % (137 of 146)	96.7 % (204 of 211)	95.7 % (66 of 69)
Sidechain	63.3 % (318 of 502)	57.9 % (176 of 304)	71.0 % (137 of 193)	100.0 % (5 of 5)
Aromatic	37.5 % (18 of 48)	45.8 % (11 of 24)	29.2 % (7 of 24)
Methyl	63.8 % (60 of 94)	61.7 % (29 of 47)	66.0 % (31 of 47)

1. entity

MAKDDVIQMQ GEVIENLPNA TFRVKLENGH VVLGHISGKM RMHYIRILPG DKVTVELTPY DLSRARIVFR AK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2N3S, Strand ID: A Detail

Release date

2015-06-22

Related entities 1. Translation initiation factor IF-1 from Burkholderia thailandensis E264, : 1 : 1 : 3 : 2 : 130 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Translation initiation factor IF-1	[U-95% 13C; U-95% 15N]		1.2 mM

nullKeywords Burkholderia thailandensis, NMR, Translation initiation factor