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BMRB: 25663

2N40

SOLUTION STRUCTURE OF THE LINK MODULE OF HUMAN TSG-6 IN PRESENCE OF A CHONDROITIN 4-SULFATE HEXASACCHARIDE

Authors

Park, Y., Prestegard, J.H.

Assembly

Link HUMAN TSG-6

Entity

1. Link HUMAN TSG-6 (polymer, Thiol state: all disulfide bound), 98 monomers, 10926.41 Da Detail

GVYHREARSG KYKLTYAEAK AVCEFEGGHL ATYKQLEAAR KIGFHVCAAG WMAKGRVGYP IVKPGPNCGF GKTGIIDYGI RLNRSERWDA YCYNPHAK

Formula weight

10926.41 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS23:SG	1:CYS92:SG
2	disulfide	sing	1:CYS47:SG	1:CYS68:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 89.3 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.3 % (1015 of 1136)	95.8 % (567 of 592)	80.0 % (355 of 444)	93.0 % (93 of 100)
Backbone	94.1 % (546 of 580)	96.1 % (197 of 205)	93.2 % (262 of 281)	92.6 % (87 of 94)
Sidechain	86.3 % (553 of 641)	95.6 % (370 of 387)	71.4 % (177 of 248)	100.0 % (6 of 6)
Aromatic	44.0 % (59 of 134)	85.1 % (57 of 67)	0.0 % (0 of 65)	100.0 % (2 of 2)
Methyl	97.7 % (84 of 86)	95.3 % (41 of 43)	100.0 % (43 of 43)

1. Link-TSG-6

GVYHREARSG KYKLTYAEAK AVCEFEGGHL ATYKQLEAAR KIGFHVCAAG WMAKGRVGYP IVKPGPNCGF GKTGIIDYGI RLNRSERWDA YCYNPHAK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	50 mM
2	NaN3	natural abundance	0.02 %
3	Link-TSG-6	[U-100% 13C; U-100% 15N]	0.5 mM
4	CHONDROITIN 4-SULFATE HEXASACCHARIDE	natural abundance	0.6 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details polyacrylamide neutral gel for an alignment media

#	Name	Isotope labeling	Concentration
5	MES	natural abundance	50 mM
6	NaN3	natural abundance	0.02 %
7	Link-TSG-6	[U-100% 13C; U-100% 15N]	0.5 mM
8	CHONDROITIN 4-SULFATE HEXASACCHARIDE	natural abundance	1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	external	indirect	1.0

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	external	indirect	1.0

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	external	indirect	1.0

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	external	indirect	1.0

Referencing offset: 0.19 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N40, Strand ID: A Detail

Release date

2016-01-10

Citation

Nuclear Magnetic Resonance Insight into the Multiple Glycosaminoglycan Binding Modes of the Link Module from Human TSG-6
Park, Y., Jowitt, T.A., Day, A.J., Prestegard, J.H.
Biochemistry (2016), 55, 262-276, PubMed 26685054 , DOI 10.1021/acs.biochem.5b01148 , Abstract

Related entities 1. Link HUMAN TSG-6, : 1 : 5 : 1 : 225 entities Detail

Interaction partners 1. Link HUMAN TSG-6, : 26 interactors Detail

More details for 26 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC