BMRB: 25671

1H Chemical Shift Assignments of the HIV ISS element

Authors

Tolbert, B.S., Jain, N., Morgan, C.E., Rife, B.D., Salemi, M.

Assembly

HIV ISS element

Entity

1. HIV ISS element (polymer, Thiol state: not present), 53 monomers, 17068.97 Da Detail

GGAAUAUUUU UGCUGUACUU UCUAUAGUGA AUAGAGUUAG GCAGGGAUAU UCC

Formula weight

17068.97 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 37.7 %, Completeness (bb): 33.0 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	37.7 % (172 of 456)	37.7 % (172 of 456)
Suger, PO4	33.0 % (105 of 318)	33.0 % (105 of 318)
Nucleobase	48.6 % (67 of 138)	48.6 % (67 of 138)
Aromatic	53.2 % (67 of 126)	53.2 % (67 of 126)

1. RNA (53-MER)

GGAAUAUUUU UGCUGUACUU UCUAUAGUGA AUAGAGUUAG GCAGGGAUAU UCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	ISS (53-MER)	[U-2H]		80.0 ~ 120.0 uM
2	D2O	natural abunance		100 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	ISS (53-MER)	[U-2H]		80.0 ~ 120.0 uM
4	D2O	natural abunance		100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	ISS (53-MER)	[U-100% 13C]		80.0 ~ 120.0 uM
6	D2O	natural abunance		100 %

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Release date

2015-11-29

Citation

Solution Structure of the HIV-1 Intron Splicing Silencer and its Interactions with the UP1 Domain of hnRNP A1
Jain, N., Morgan, C.E., Rife, B.D., Salemi, M., Tolbert, B.S.
J. Biol. Chem. (2015), 291, 2331-2344, PubMed 26607354 , DOI 10.1074/jbc.M115.674564 , Abstract

Experiments performed 3 experiments Detail