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BMRB: 25677

2N4Q

Solution NMR structure of CBX8 in complex with AF9 (CBX8-AF9)

Authors

Kuntimaddi, A., Leach, B.I., Bushweller, J.H.

Assembly

CBX8 in complex with AF9

Entity

1. Chromobox protein homolog 8 (polymer, Thiol state: not present), 24 monomers, 2574.886 Da Detail

TQGGRPSLIA RIPVARILGD PEEE

2. Protein AF-9 (polymer), 70 monomers, 8266.313 Da Detail

MDKAYLDELV ELHRRLMTLR ERHILQQIVN LIEETGHFHI TNTTFDFDLC SLDKTTVRKL QSYLETSGTS

Total weight

10841.199 Da

Max. entity weight

8266.313 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 86.6 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.6 % (957 of 1105)	80.5 % (462 of 574)	93.8 % (407 of 434)	90.7 % (88 of 97)
Backbone	95.3 % (532 of 558)	93.7 % (178 of 190)	96.0 % (266 of 277)	96.7 % (88 of 91)
Sidechain	80.7 % (513 of 636)	74.0 % (284 of 384)	93.1 % (229 of 246)	0.0 % (0 of 6)
Aromatic	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)
Methyl	77.9 % (95 of 122)	73.8 % (45 of 61)	82.0 % (50 of 61)

1. Chromobox protein homolog 8

TQGGRPSLIA RIPVARILGD PEEE

2. Protein AF-9

MDKAYLDELV ELHRRLMTLR ERHILQQIVN LIEETGHFHI TNTTFDFDLC SLDKTTVRKL QSYLETSGTS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	Chromobox protein homolog 8	[U-100% 13C; U-100% 15N]	750 uM
2	Protein AF-9	[U-100% 13C; U-100% 15N]	750 uM
3	BIS-TRIS	natural abundance	9.5 mM
4	MES	natural abundance	15.8 mM
5	sodium chloride	natural abundance	100 mM
6	DTT	natural abundance	1 mM
7	D2O	natural abundance	5 %
8	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N4Q, Strand ID: A, B Detail

Release date

2016-07-17

Citation

Solution NMR Structure of CBX8 in complex with AF9 (CBX8-AF9)
Kuntimaddi, A., Leach, B.I., Bushweller, J.H.
Not known

Related entities 1. Chromobox protein homolog 8, : 5 : 2 entities Detail

Related entities 2. Protein AF-9, : 3 : 16 entities Detail

Interaction partners 1. Chromobox protein homolog 8, : 8 interactors Detail

More details for 8 interactors identified by 3 citations

Interaction partners 2. Protein AF-9, : 29 : 1 interactors Detail

More details for 30 interactors identified by 16 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC