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BMRB: 25686


25686
2N4Y
Structure of a G-quadruplex in the Long Terminal Repeat of the proviral HIV-1 genome
Authors
De Nicola, B., Lech, C., Regmi, S., Heddi, B., Richter, S., Phan, A.
Assembly
G-quadruplex in the Long Terminal Repeat of the proviral HIV-1 genome
Entity
1. G-quadruplex in the Long Terminal Repeat of the proviral HIV-1 genome (polymer, Thiol state: not present), 22 monomers, 7008.419 Da Detail

CTGGGCGGGA CTGGGGAGTG GT


Formula weight
7008.419 Da
Source organism
Human immunodeficiency virus
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 94.7 %, Completeness (bb): 100.0 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All94.7 % (197 of 208)94.7 % (197 of 208)
Suger, PO4100.0 % (154 of 154)100.0 % (154 of 154)
Nucleobase79.6 % (43 of 54)79.6 % (43 of 54)
Aromatic88.6 % (39 of 44)88.6 % (39 of 44)
Methyl100.0 % (4 of 4)100.0 % (4 of 4)

1. DNA (5'-D(*CP*TP*GP*GP*GP*CP*GP*GP*GP*AP*CP*TP*GP*GP*GP*GP*AP*GP*TP*GP*GP*T)-3')

CTGGGCGGGA CTGGGGAGTG GT

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*CP*TP*GP*GP*GP*CP*GP*GP*GP*AP*CP*TP*GP*GP*GP*GP*AP*GP*TP*GP*GP*T)-3')natural abundance0.1 ~ 1.0 mM
2K+natural abundance10 mM
3H2Onatural abundance90 %
4D2Onatural abundance10 %

Release date
2016-06-26
Citation
Structure and possible function of a G-quadruplex in the long terminal repeat of the proviral HIV-1 genome
de Nicola, B., Lech, C., Regmi, S., Heddi, B., Richter, S., Phan, A.
Nucleic Acids Res. (2016), 44, 6442-6451, PubMed 27298260 , DOI 10.1093/nar/gkw432 ,
Experiments performed 4 experiments Detail
NMR combined restraints 6 contents Detail
Keywords DNA, G-quadruplex, HIV-1, Long Terminal Repeat of HIV-1 genome