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BMRB: 25734

5AAQ

TBK1 recruitment to cytosol-invading Salmonella induces anti-bacterial autophagy

Authors

Thurston, T.L., Allen, M.D., Ravenhill, B., Karpiyevitch, M., Bloor, S., Kaul, A., Matthews, S., Komander, D., Holden, D., Bycroft, M., Randow, F., Matthews, A.

Assembly

TBK1 recruitment to cytosol-invading Salmonella induces anti-bacterial autophagy

Entity

1. CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING 2 (polymer, Thiol state: free and disulfide bound), 60 monomers, 6792.828 Da Detail

GSPSPLSIKK CPICKADDIC DHTLEQQQMQ PLCFNCPICD KIFPATEKQI FEDHVFCHSL

2. ZINC ION (non-polymer), 65.409 Da

Total weight

6858.2373 Da

Max. entity weight

6792.828 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 78.1 %, Completeness (bb): 88.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.1 % (554 of 709)	88.7 % (331 of 373)	63.0 % (174 of 276)	81.7 % (49 of 60)
Backbone	88.8 % (309 of 348)	88.7 % (102 of 115)	88.8 % (159 of 179)	88.9 % (48 of 54)
Sidechain	71.0 % (298 of 420)	88.8 % (229 of 258)	43.6 % (68 of 156)	16.7 % (1 of 6)
Aromatic	48.1 % (25 of 52)	96.2 % (25 of 26)	0.0 % (0 of 26)
Methyl	67.3 % (35 of 52)	84.6 % (22 of 26)	50.0 % (13 of 26)

1. CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING 2

GSPSPLSIKK CPICKADDIC DHTLEQQQMQ PLCFNCPICD KIFPATEKQI FEDHVFCHSL

Show sample condition

Sample

Solvent system 95% water/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1.5 mmol/l

#	Name	Isotope labeling	Concentration
1	CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING 2	[U-13C; U-15N]	1.5 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	1.0
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5AAQ, Strand ID: A Detail

Release date

2016-07-10

Citation

Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy
Thurston, T.L., Boyle, K.B., Allen, M., Ravenhill, B.J., Karpiyevich, M., Bloor, S., Kaul, A., Noad, J., Foeglein, A., Matthews, S.A., Komander, D., Bycroft, M., Randow, F.
EMBO J. (2016), 35, 1779-1792, PubMed 27370208 , DOI 10.15252/embj.201694491 , Abstract

Related entities 1. CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING 2, : 4 : 13 entities Detail

Interaction partners 1. CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING 2, : 149 interactors Detail

More details for 149 interactors identified by 16 citations

Experiments performed 9 experiments Detail

1 NOESY