BMRBj - BMREntryMaster

Found 1 Document (7.102 seconds)

BMRB: 25735

5AAS

The selective autophagy receptor TAX1BP1 is required for autophagy-dependent capture of cytosolic Salmonella typhimurium

Authors

Tumbarello, D.A., Manna, P.T., Allen, M.D., Bycroft, M., Kendrick-jones, J., Buss, F.

Assembly

The selective autophagy receptor TAX1BP1 is required for autophagy-dependent capture of cytosolic Salmonella typhimurium

Entity

1. TAXBP1 (polymer, Thiol state: all disulfide bound), 64 monomers, 7688.444 Da Detail

GSSFDVHKKC PLCELMFPPN YDQSKFEEHV ESHWKVCPMC SEQFPPDYDQ QVFERHVQTH FDQN

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

7819.2617 Da

Max. entity weight

7688.444 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 83.7 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.7 % (655 of 783)	97.1 % (401 of 413)	63.0 % (191 of 303)	94.0 % (63 of 67)
Backbone	94.1 % (350 of 372)	94.3 % (116 of 123)	94.2 % (180 of 191)	93.1 % (54 of 58)
Sidechain	77.0 % (365 of 474)	98.3 % (285 of 290)	40.6 % (71 of 175)	100.0 % (9 of 9)
Aromatic	53.7 % (58 of 108)	100.0 % (54 of 54)	5.7 % (3 of 53)	100.0 % (1 of 1)
Methyl	56.7 % (17 of 30)	100.0 % (15 of 15)	13.3 % (2 of 15)

1. TAXBP1

GSSFDVHKKC PLCELMFPPN YDQSKFEEHV ESHWKVCPMC SEQFPPDYDQ QVFERHVQTH FDQN

Show sample condition

Sample

Solvent system 95% water/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1.5 mmol/l

#	Name	Isotope labeling	Concentration
1	TAXBP1	[U-13C; U-15N]	1.5 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	1.0
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	1.0
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	1.0
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	1.0
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	Liquid NH3	NH3	external	indirect	1.0

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5AAS, Strand ID: A Detail

Release date

2015-09-20

Citation

The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy
Tumbarello, D.A., Manna, P.T., Allen, M., Bycroft, M., Arden, S.D., Kendrick-Jones, J., Buss, F.
PLoS Pathog. (2015), 11, e1005174-e1005174, PubMed 26451915 , DOI 10.1371/journal.ppat.1005174 , Abstract

Related entities 1. TAXBP1, : 1 : 8 : 43 entities Detail

Interaction partners 1. TAXBP1, : 63 interactors Detail

More details for 63 interactors identified by 17 citations

Experiments performed 9 experiments Detail

1 NOESY