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BMRB: 25744

2N5Z

NMR structure and dynamics of the resuscitation promoting factor RpfC catalytic domain

Authors

Maione, V., Russo, L., Isernia, C.

Assembly

RpfC

Entity

1. RpfC (polymer, Thiol state: all disulfide bound), 82 monomers, 8175.901 Da Detail

GAMGPSPNWD AVAQCESGGN WAANTGNGKY GGLQFKPATW AAFGGVGNPA AASREQQIAV ANRVLAEQGL DAWPTCGAAS GL

Formula weight

8175.901 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS15:SG	1:CYS76:SG

Source organism

Mycobacterium tuberculosis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 82.7 %, Completeness (bb): 81.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.7 % (700 of 846)	88.7 % (383 of 432)	75.5 % (243 of 322)	80.4 % (74 of 92)
Backbone	81.1 % (391 of 482)	93.1 % (161 of 173)	67.7 % (157 of 232)	94.8 % (73 of 77)
Sidechain	86.6 % (374 of 432)	85.7 % (222 of 259)	95.6 % (151 of 158)	6.7 % (1 of 15)
Aromatic	88.2 % (67 of 76)	89.5 % (34 of 38)	94.1 % (32 of 34)	25.0 % (1 of 4)
Methyl	94.7 % (72 of 76)	94.7 % (36 of 38)	94.7 % (36 of 38)

1. entity

GAMGPSPNWD AVAQCESGGN WAANTGNGKY GGLQFKPATW AAFGGVGNPA AASREQQIAV ANRVLAEQGL DAWPTCGAAS GL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
4	entity	[U-100% 13C; U-100% 15N]	1 mM
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N5Z, Strand ID: A Detail

Release date

2015-11-16

Citation

NMR Structure and Dynamics of the Resuscitation Promoting Factor RpfC Catalytic Domain
Maione, V., Ruggiero, A., Russo, L., De Simone, A., Pedone, P.VINCENZO., Malgieri, G., Berisio, R., Isernia, C.
PLoS One (2015), 10, e0142807-e0142807, PubMed 26576056 , DOI 10.1371/journal.pone.0142807 , Abstract

Related entities 1. RpfC, : 1 : 4 : 22 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC