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BMRB: 25746


25746
2N60
G-quadruplex structure
Authors
Heddi, B., Martin-Pintado, N., Serimbetov, Z., Kari, T., Phan, A.
Assembly
DNA G-quadruplex
Entity
1. DNA G-quadruplex (polymer, Thiol state: not present), 18 monomers, 5768.627 Da Detail

TTGTGTGGGT GGGTGGGT


Formula weight
5768.627 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 83.3 %, Completeness: 62.7 %, Completeness (bb): 61.1 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All62.7 % (106 of 169)62.7 % (106 of 169)
Suger, PO461.1 % (77 of 126)61.1 % (77 of 126)
Nucleobase67.4 % (29 of 43)67.4 % (29 of 43)
Aromatic69.4 % (25 of 36)69.4 % (25 of 36)
Methyl57.1 % (4 of 7)57.1 % (4 of 7)

1. DNA (5'-D(*TP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3')

TTGTGTGGGT GGGTGGGT

Show sample condition
Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*TP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3')natural abundance0.1 ~ 1.2 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
4DNA (5'-D(*TP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3')[U-4% 15N]0.2-0.4 mM
5H2Onatural abundance90 %
6D2Onatural abundance10 %
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
7DNA (5'-D(*TP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3')natural abundance0.6-1.2 mM
8D2Onatural abundance100 %
Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
9DNA (5'-D(*TP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3')[U-100% 2H]0.2-0.4 mM
10H2Onatural abundance90 %
11D2Onatural abundance10 %

Release date
2016-05-30
Citation
G-quadruplexes with (4n - 1) guanines in the G-tetrad core: formation of a G-triad·water complex and implication for small-molecule binding
Heddi, B., Martin-Pintado, N., Serimbetov, Z., Kari, T., Phan, A.
Nucleic Acids Res. (2016), 44, 910-916, PubMed 26673723 , DOI 10.1093/nar/gkv1357 ,
Experiments performed 6 experiments Detail
NMR combined restraints 7 contents Detail
Keywords 11 guanines, G-quadruplex, G-triad, K+ solution, Vacant site