ddFLN5+110
HHHHHHASKP APSAEHSYAE GEGLVKVFDN APAEFTIFAV DTKGVARTDG GDPFEVAING PDGLVVDAKV TDNNDGTYGV VYDAPVEGNY NVNVTLRGNP IKNMPIDVKC IEGANGEDSS FGSFTFTVAA KNKKGEVKTY GGDKFEVSIT GPAEEITLDA IDNQDGTYTA AYSLVGNGRF STGVKLNGKH IEGSPFKQVL GNELFSTPVW ISQAQGIRAG P
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 29.5 % (713 of 2414) | 21.3 % (263 of 1237) | 33.9 % (321 of 948) | 56.3 % (129 of 229) |
Backbone | 49.4 % (643 of 1302) | 47.0 % (215 of 457) | 47.3 % (301 of 636) | 60.8 % (127 of 209) |
Sidechain | 12.0 % (157 of 1306) | 5.9 % (46 of 780) | 21.7 % (110 of 506) | 5.0 % (1 of 20) |
Aromatic | 3.8 % (8 of 210) | 3.8 % (4 of 105) | 3.8 % (4 of 104) | 0.0 % (0 of 1) |
Methyl | 13.9 % (32 of 230) | 11.3 % (13 of 115) | 16.5 % (19 of 115) |
1. entity
HHHHHHASKP APSAEHSYAE GEGLVKVFDN APAEFTIFAV DTKGVARTDG GDPFEVAING PDGLVVDAKV TDNNDGTYGV VYDAPVEGNY NVNVTLRGNP IKNMPIDVKC IEGANGEDSS FGSFTFTVAA KNKKGEVKTY GGDKFEVSIT GPAEEITLDA IDNQDGTYTA AYSLVGNGRF STGVKLNGKH IEGSPFKQVL GNELFSTPVW ISQAQGIRAG PSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ddFLN5+110 | [U-15N] | 10 uM | |
2 | HEPES | natural abundance | 10 mM | |
3 | ammonium chloride | natural abundance | 30 mM | |
4 | MgCl2 | natural abundance | 12 mM | |
5 | BME | natural abundance | 2 mM | |
6 | EDTA | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | ddFLN5+110 | [U-100% 2H, Ile d1-13CH3] | 10 uM | |
8 | HEPES | [U-2H] | 10 mM | |
9 | ammonium chloride | [U-2H] | 30 mM | |
10 | MgCl2 | [U-2H] | 12 mM | |
11 | BME | [U-2H] | 2 mM | |
12 | EDTA | [U-2H] | 1 mM |