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BMRB: 25762

2N6F

Structure of Pleiotrophin

Authors

Ryan, E.O., Shen, D., Wang, X.

Assembly

Pleiotrophin

Entity

1. Pleiotrophin (polymer), 136 monomers, 15312.65 Da Detail

GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD

Formula weight

15312.65 Da

Entity Connection

disulfide 5 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS15:SG	1:CYS44:SG
2	disulfide	sing	1:CYS23:SG	1:CYS53:SG
3	disulfide	sing	1:CYS30:SG	1:CYS57:SG
4	disulfide	sing	1:CYS67:SG	1:CYS99:SG
5	disulfide	sing	1:CYS77:SG	1:CYS109:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 76.7 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.7 % (1251 of 1632)	66.2 % (581 of 877)	89.0 % (542 of 609)	87.7 % (128 of 146)
Backbone	93.0 % (748 of 804)	89.9 % (250 of 278)	94.7 % (375 of 396)	94.6 % (123 of 130)
Sidechain	65.5 % (624 of 952)	55.4 % (332 of 599)	85.2 % (287 of 337)	31.3 % (5 of 16)
Aromatic	77.5 % (62 of 80)	77.5 % (31 of 40)	75.0 % (27 of 36)	100.0 % (4 of 4)
Methyl	91.1 % (82 of 90)	91.1 % (41 of 45)	91.1 % (41 of 45)

1. PTN

GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	PTN	[U-100% 13C; U-100% 15N]	0.6 (±0.05) mM
2	D2O	natural abundance	10 %
3	MES	natural abundance	10 mM
4	sodium chloride	natural abundance	150 mM
5	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N6F, Strand ID: A Detail

Release date

2016-03-28

Citation

Structural Studies Reveal an Important Role for the Pleiotrophin C Terminus in Mediating Interactions with Chondroitin Sulfate
Ryan, E.O., Shen, D., Wang, X.
FEBS J. (2016), 283, 1488-1503, PubMed 26896299 , DOI 10.1111/febs.13686 , Abstract

Related entities 1. Pleiotrophin, : 1 : 2 : 4 : 4 entities Detail

Interaction partners 1. Pleiotrophin, : 88 interactors Detail

More details for 88 interactors identified by 4 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC