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BMRB: 25763

2N6G

Solution structure of an MbtH-like protein from Mycobacterium avium, Seattle Structural Genomics Center for Infectious Disease target MyavA.01649.c

Authors

Buchko, G.W.

Assembly

MbtH-like protein from Mycobacterium avium

Entity

1. MbtH-like protein from Mycobacterium avium (polymer), 80 monomers, 8984.753 Da Detail

GPGSMSINPF DDDNGSFFVL VNDEEQHSLW PAFADVPAGW RVVHGEADRA ACLEYIEEHW PDIRPKSLRD KLATGRGFDQ

Formula weight

8984.753 Da

Source organism

Mycolicibacterium gadium

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 66.6 %, Completeness (bb): 74.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.6 % (606 of 910)	61.2 % (289 of 472)	71.3 % (254 of 356)	76.8 % (63 of 82)
Backbone	74.6 % (349 of 468)	69.6 % (112 of 161)	76.8 % (179 of 233)	78.4 % (58 of 74)
Sidechain	60.8 % (313 of 515)	56.9 % (177 of 311)	66.8 % (131 of 196)	62.5 % (5 of 8)
Aromatic	44.3 % (47 of 106)	45.3 % (24 of 53)	40.0 % (20 of 50)	100.0 % (3 of 3)
Methyl	88.2 % (60 of 68)	82.4 % (28 of 34)	94.1 % (32 of 34)

1. entity

GPGSMSINPF DDDNGSFFVL VNDEEQHSLW PAFADVPAGW RVVHGEADRA ACLEYIEEHW PDIRPKSLRD KLATGRGFDQ

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	sodium chloride	natural abundance	100 (±3.0) mM
2	TRIS	natural abundance	20 (±0.5) mM
3	DTT	natural abundance	1 (±0.01) mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N6G, Strand ID: A Detail

Release date

2015-12-27

Citation

Solution structure of a MbtH-like protein from Mycobacterium avium
Buchko, G.W., Hewitt, S.N., Van Voorhis, W.C., Myler, P.J.
Not known

Related entities 1. MbtH-like protein from Mycobacterium avium, : 1 : 26 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC