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BMRB: 25768

2N6L

Solution NMR structure of Outer Membrane Protein G from Pseudomonas aeruginosa

Authors

Kucharska, I., Seelheim, P., Edrington, T.C., Liang, B., Tamm, L.K.

Assembly

Outer Membrane Protein G from Pseudomonas aeruginosa

Entity

1. Outer Membrane Protein G from Pseudomonas aeruginosa (polymer, Thiol state: not present), 215 monomers, 23688.39 Da Detail

MHKAGDFIIR GGFATVDPDD SSSDIKLDGA KQRGTKATVD SDTQLGLTFT YMFADKWGVE LVAATPFNHQ VDVKGLGPGL DGKLADIKQL PPTLLLQYYP MGGTNSAFQP YGGLGVNYTT FFDEDLASNR KAQGFSSMKL QDSWGLAGEL GFDYMLNEHA LFNMAVWYMD IDTKASINGP SALGVNKTKV DVDVDPWVYM IGFGYKFLEH HHHHH

Formula weight

23688.39 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 42.5 %, Completeness (bb): 75.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.5 % (1048 of 2466)	22.7 % (287 of 1265)	59.5 % (580 of 975)	80.1 % (181 of 226)
Backbone	75.1 % (955 of 1272)	47.9 % (213 of 445)	90.3 % (561 of 621)	87.9 % (181 of 206)
Sidechain	19.0 % (263 of 1385)	8.9 % (73 of 820)	34.9 % (190 of 545)	0.0 % (0 of 20)
Aromatic	5.2 % (15 of 286)	7.0 % (10 of 143)	3.6 % (5 of 139)	0.0 % (0 of 4)
Methyl	19.7 % (43 of 218)	18.3 % (20 of 109)	21.1 % (23 of 109)

1. entity

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N; U-2H]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.05 %
5	1,2-dihexanoyl-sn-glycero-3-phosphocholine	natural abundance	0.9 %
6	D2O	natural abundance	10 %
7	H2O	natural abundance	90 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	entity	[U-13C; U-15N; U-2H]	1 mM
9	sodium phosphate	natural abundance	25 mM
10	potassium chloride	natural abundance	50 mM
11	sodium azide	natural abundance	0.05 %
12	1,2-dihexanoyl-sn-glycero-3-phosphocholine	natural abundance	0.9 %
13	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N6L, Strand ID: A Detail

Release date

2015-12-27

Citation

OprG Harnesses the Dynamics of its Extracellular Loops to Transport Small Amino Acids across the Outer Membrane of Pseudomonas aeruginosa
Kucharska, I., Seelheim, P., Edrington, T.C., Liang, B., Tamm, L.K.
Structure (2015), 23, 2234-2245, PubMed 26655471 , DOI 10.1016/j.str.2015.10.009 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25776 released on 2015-12-27
Title Solution NMR structure of Outer Membrane Protein G P92A mutant from Pseudomonas aeruginosa