BMRBj - BMREntryMaster

Found 1 Document (1.111 seconds)

BMRB: 25794

2N75

Solution NMR Structure of DE NOVO DESIGNED PROTEIN, Rossmann2x2 Fold, Northeast Structural Genomics Consortium (NESG) Target OR446

Authors: Liu, G., Lin, Y., Koga, N., Koga, R., Xiao, R., Janjua, H., Pederson, K., Acton, T.B., Kornhaber, G., Everett, J.K., Baker, D., Montelione, G.T.
Assembly: NESG Target OR446
Entity: 1. NESG Target OR446 (polymer, Thiol state: not present), 99 monomers, 11353.96 Da Detail
MGRLVVVVTS EQLKEEVRKK FPQVEVRLVT TEEDAKQVIK EIQKKGVQKV VLVGVSEKLL QKIKQEANVQ VYRVTSNDEL EQVVKDVKGS GLEHHHHHH
Formula weight: 11353.96 Da
Source organism: Other
Exptl. method: solution NMR
Refine. method: molecular dynamics, simulated annealing
Data set: RDCs
Protein Blocks Logo: Calculated from 20 models in PDB: 2N75, Strand ID: A Detail
RDC: 136 RDC values in 2 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail
Pressure 1 atm, Temperature 298 K, pH 6.5
Experiment name 2D 1H-15N HSQC
List #1 RDC_list_1, RDC code 1DHN, Field strength (¹H) 600 MHz
List #2 RDC_list_2, RDC code 1DHN, Field strength (¹H) 600 MHz
Release date: 2016-01-24

Related entities 1. NESG Target OR446, : 1 : 1 : 13 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

3 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

5 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

6 3D 1H-13C arom NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

7 3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.34 mM OR446.003, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR446.003	[U-100% 13C; U-100% 15N]	1.34 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.8 mM

Keywords NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-Biology, Protein NMR, Protein Structure Initiative, Structural Genomics, Target OR446

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Found 1 Document (1.111 seconds)

BMRB: 25794

Related entities 1. NESG Target OR446, : 1 : 1 : 13 entities Detail

Experiments performed 7 experiments Detail

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