BMRB: 25797

The structural and functional effects of the Familial Hypertrophic Cardiomyopathy-linked cardiac troponin C mutation, L29Q

Authors

Robertson, I.M., Sevrieva, I., Li, M.X., Irving, M., Sun, Y., Sykes, B.

Assembly

cardiac troponin C

Entity

1. cardiac troponin C, entity 1 (polymer, Thiol state: not present), 89 monomers, 10045.07 Da Detail

MDDIYKAAVE QLTEEQKNEF KAAFDIFVQG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDS

2. CALCIUM ION (non-polymer), 40.078 Da

Total weight

10085.148 Da

Max. entity weight

10045.07 Da

Entity Connection

na 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:ASP65:OD1	2:CA1:CA
2	na	sing	1:ASP67:OD1	2:CA1:CA
3	na	sing	1:SER69:OG	2:CA1:CA
4	na	sing	1:THR71:O	2:CA1:CA
5	na	sing	1:GLU76:OE1	2:CA1:CA
6	na	sing	1:GLU76:OE2	2:CA1:CA

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.4 %, Completeness (bb): 85.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.4 % (857 of 1015)	88.7 % (469 of 529)	75.5 % (296 of 392)	97.9 % (92 of 94)
Backbone	85.1 % (451 of 530)	98.9 % (180 of 182)	70.9 % (185 of 261)	98.9 % (86 of 87)
Sidechain	86.1 % (489 of 568)	83.3 % (289 of 347)	90.7 % (194 of 214)	85.7 % (6 of 7)
Aromatic	51.7 % (30 of 58)	62.1 % (18 of 29)	41.4 % (12 of 29)
Methyl	97.6 % (80 of 82)	97.6 % (40 of 41)	97.6 % (40 of 41)

1. entity 1

MDDIYKAAVE QLTEEQKNEF KAAFDIFVQG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-95% 13C; U-95% 15N]	protein	0.3 ~ 0.4 mM
2	H2O	natural abundance	solvent	95 %
3	D2O	[U-2H]	solvent	5 %

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2N79, Strand ID: C Detail

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Release date

2015-11-16

Citation

The structural and functional effects of the familial hypertrophic cardiomyopathy-linked cardiac troponin C mutation, L29Q
Robertson, I., Sevrieva, I., Li, M., Irving, M., Sun, Y., Sykes, B.
J. Mol. Cell. Cardiol. (2015), 87, 257-269, PubMed 26341255 , DOI 10.1016/j.yjmcc.2015.08.017 , Abstract

Related entities 1. cardiac troponin C, entity 1, : 1 : 48 : 268 entities Detail

Interaction partners 1. cardiac troponin C, entity 1, : 8 interactors Detail

Experiments performed 12 experiments Detail