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BMRB: 25802

2NAN

Resonance assignments of [15N, 13C] human DCL-1 (CD302) extracellular domain

Authors

Pospisilova, E., Kavan, D., Novak, P., Chmelik, J.

Assembly

CD302

Entity

1. CD302 (polymer, Thiol state: all disulfide bound), 140 monomers, 16211.83 Da Detail

MDCPSSTWIQ FQDSCYIFLQ EAIKVESIED VRNQCTDHGA DMISIHNEEE NAFILDTLKK QWKGPDDILL GMFYDTDDAS FKWFDNSNMT FDKWTDQDDD EDLVDTCAFL HIKTGEWKKG NCEVSSVEGT LCKTAIPYKR

Formula weight

16211.83 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 97.9 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.9 % (1606 of 1640)	97.9 % (830 of 848)	98.0 % (625 of 638)	98.1 % (151 of 154)
Backbone	98.0 % (817 of 834)	97.9 % (277 of 283)	98.1 % (406 of 414)	97.8 % (134 of 137)
Sidechain	98.1 % (922 of 940)	97.9 % (553 of 565)	98.3 % (352 of 358)	100.0 % (17 of 17)
Aromatic	96.6 % (170 of 176)	96.6 % (85 of 88)	96.4 % (80 of 83)	100.0 % (5 of 5)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. CD302

MDCPSSTWIQ FQDSCYIFLQ EAIKVESIED VRNQCTDHGA DMISIHNEEE NAFILDTLKK QWKGPDDILL GMFYDTDDAS FKWFDNSNMT FDKWTDQDDD EDLVDTCAFL HIKTGEWKKG NCEVSSVEGT LCKTAIPYKR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CD302	[U-100% 13C; U-100% 15N]	0.5 mM
2	PIPES	natural abundance	25 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2NAN, Strand ID: A Detail

Release date

2016-01-05

Citation

¹H, ¹³C and ¹⁵N resonance assignments of human DCL-1 (CD302) extracellular domain
Pospisilova, E., Kavan, D., Novak, P., Chmelik, J.
Biomol. NMR Assign. (2016), 10, 189-192, PubMed 26725057 , DOI 10.1007/s12104-015-9664-5 , Abstract

Related entities 1. CD302, : 1 : 2 : 287 entities Detail

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC