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BMRB: 25805

2N7G

Structure of the cyclic nucleotide-binding homology domain of the hERG channel

Authors

Li, Y., Ng, H., Li, Q., Kang, C.

Assembly

cyclic nucleotide-binding homology domain of hERG channel

Entity

1. cyclic nucleotide-binding homology domain of hERG channel (polymer, Thiol state: all free), 154 monomers, 17411.69 Da Detail

MHHHHHHSSG VDLGTENLYF QSMRSLLQHC KPFRGATKGC LRALAMKFKT THAPPGDTLV HAGDLLTALY FISRGSIEIL RGDVVVAILG KNDIFGEPLN LYARPGKSNG DVRALTYCDL HKIHRDDLLE VLDMYPEFSD HFWSSLEITF NLRD

Formula weight

17411.69 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.3 %, Completeness: 76.0 %, Completeness (bb): 85.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.0 % (1361 of 1791)	75.5 % (697 of 923)	74.9 % (533 of 712)	84.0 % (131 of 156)
Backbone	85.5 % (780 of 912)	83.4 % (262 of 314)	86.4 % (389 of 450)	87.2 % (129 of 148)
Sidechain	69.0 % (705 of 1021)	71.4 % (435 of 609)	66.3 % (268 of 404)	25.0 % (2 of 8)
Aromatic	23.3 % (42 of 180)	38.9 % (35 of 90)	6.7 % (6 of 89)	100.0 % (1 of 1)
Methyl	87.4 % (152 of 174)	88.5 % (77 of 87)	86.2 % (75 of 87)

1. the cyclic nucleotide-binding homology domain of the hERG channel

MHHHHHHSSG VDLGTENLYF QSMRSLLQHC KPFRGATKGC LRALAMKFKT THAPPGDTLV HAGDLLTALY FISRGSIEIL RGDVVVAILG KNDIFGEPLN LYARPGKSNG DVRALTYCDL HKIHRDDLLE VLDMYPEFSD HFWSSLEITF NLRD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	the cyclic nucleotide-binding homology domain of the hERG channel	[U-100% 13C; U-100% 15N]	protein	0.4 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	150 mM
4	DTT	natural abundance		1 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.73 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.73 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N7G, Strand ID: A Detail

Release date

2016-05-22

Citation

Structure of the Cyclic Nucleotide-Binding Homology Domain of the hERG Channel and Its Insight into Type 2 Long QT Syndrome
Li, Y., Ng, H., Li, Q., Kang, C.
Sci. Rep. (2016), 6, 23712-23712, PubMed 27025590 , DOI 10.1038/srep23712 , Abstract

Related entities 1. cyclic nucleotide-binding homology domain of hERG channel, : 1 : 14 : 54 entities Detail

Interaction partners 1. cyclic nucleotide-binding homology domain of hERG channel, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC