Structure of the transmembrane domain of human nicastrin in SDS micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 60.9 % (382 of 627) | 64.6 % (204 of 316) | 51.9 % (134 of 258) | 83.0 % (44 of 53) |
Backbone | 82.5 % (264 of 320) | 84.4 % (92 of 109) | 80.5 % (128 of 159) | 84.6 % (44 of 52) |
Sidechain | 43.9 % (157 of 358) | 54.1 % (112 of 207) | 30.0 % (45 of 150) | 0.0 % (0 of 1) |
Aromatic | 5.7 % (4 of 70) | 11.4 % (4 of 35) | 0.0 % (0 of 35) | |
Methyl | 47.6 % (39 of 82) | 73.2 % (30 of 41) | 22.0 % (9 of 41) |
1. transmembrane domain of human nicastrin
MAHHHHHHAS KELELITLTV GFGILIFSLI VTYCINAKAD VLFIAPREPG AVSYSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | transmembrane domain of human nicastrin | [U-100% 13C; U-100% 15N] | 0.8 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | SDS | natural abundance | 100 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |