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BMRB: 25825

2NBW

UBL protein

Authors

Chen, X., Walters, K.J.

Assembly

UBL protein

Entity

1. UBL protein (polymer, Thiol state: all free), 99 monomers, 11020.42 × 2 Da Detail

MVSLTFKNFK KEKVPLDLEP SNTILETKTK LAQSISCEES QIKLIYSGKV LQDSKTVSEC GLKDGDQVVF MVSQKKSTDP NSSSVDKLAA ALEHHHHHH

Total weight

22040.84 Da

Max. entity weight

11020.42 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 92.0 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.0 % (1065 of 1157)	93.4 % (563 of 603)	89.8 % (404 of 450)	94.2 % (98 of 104)
Backbone	94.4 % (555 of 588)	93.9 % (186 of 198)	94.9 % (279 of 294)	93.8 % (90 of 96)
Sidechain	90.4 % (601 of 665)	93.1 % (377 of 405)	85.7 % (216 of 252)	100.0 % (8 of 8)
Aromatic	24.2 % (15 of 62)	48.4 % (15 of 31)	0.0 % (0 of 31)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. UBL protein

MVSLTFKNFK KEKVPLDLEP SNTILETKTK LAQSISCEES QIKLIYSGKV LQDSKTVSEC GLKDGDQVVF MVSQKKSTDP NSSSVDKLAA ALEHHHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UBL protein	[U-100% 13C; U-100% 15N]	0.7 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	sodium azide	natural abundance	0.1 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	UBL protein	[U-100% 13C; U-100% 15N]	0.7 mM
7	potassium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	50 mM
9	DTT	natural abundance	2 mM
10	sodium azide	natural abundance	0.1 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2NBW, Strand ID: B Detail

Release date

2016-07-17

Citation

UBL protein
Chen, X., Walters, K.J.
Not known

Related entities 1. UBL protein, : 1 : 25 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC