BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	89.6 % (1145 of 1278)	96.4 % (650 of 674)	77.7 % (377 of 485)	99.2 % (118 of 119)
Backbone	83.9 % (527 of 628)	100.0 % (211 of 211)	67.9 % (214 of 315)	100.0 % (102 of 102)
Sidechain	95.8 % (721 of 753)	94.8 % (439 of 463)	97.4 % (266 of 273)	94.1 % (16 of 17)
Aromatic	63.2 % (24 of 38)	63.2 % (12 of 19)	61.1 % (11 of 18)	100.0 % (1 of 1)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. entity

GIAQQWIQSK REDIVNQMTE ACLNQSLDAL LSRDLIMKED YELVSTKPTR TSKVRQLLDT TDIQGEEFAK VIVQKLKDNK QMGLQPYPEI LVVSRSPSLN LLQNKS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±1) atm, Temperature 301 K

#	Name	Isotope labeling	Concentration
1	CARD	[U-99% 13C; U-99% 15N]	0.8 mM
2	DTT	[U-98% 2H]	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.64 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.64 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N7Z, Strand ID: A Detail

Release date

2015-12-20

Citation

Structural basis of death domain signaling in the p75 neurotrophin receptor
Lin, Z., Tann, J.Y., Goh, E.TH., Kelly, C., Lim, K.B., Gao, J.F., Ibanez, C.F.
Elife (2015), 4, 11692-11692, PubMed 26646181 , DOI 10.7554/eLife.11692 , Abstract

Related entities 1. RIP2 CARD, : 1 : 7 : 1 : 15 entities Detail

Interaction partners 1. RIP2 CARD, : 68 interactors Detail

More details for 68 interactors identified by 14 citations

Experiments performed 7 experiments Detail

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_25828_2n7z.nef
Input source #2: Coordindates	2n7z.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
GIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLN
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLN

------
LLQNKS
||||||
LLQNKS

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	106	0	0	100.0

Content subtype: combined_25828_2n7z.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1142		100.0 (chain: A, length: 106)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	2105 (1)	noe	100.0 (chain: A, length: 106)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 106, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 648
  - ¹³C chemical shifts: 372
  - ¹⁵N chemical shifts: 122
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	674	648	96.1
¹³C chemical shifts	485	372	76.7
¹⁵N chemical shifts	124	122	98.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	211	211	100.0
¹³C chemical shifts	212	106	50.0
¹⁵N chemical shifts	102	102	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	463	437	94.4
¹³C chemical shifts	273	266	97.4
¹⁵N chemical shifts	22	20	90.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	69	100.0
¹³C chemical shifts	69	69	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	12	63.2
¹³C chemical shifts	18	11	61.1
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 106, Sequence coverage: 100.0%
- Total number of restraints: 2105
- Weight of restraints: 1.0 (2105)
- Potential type of restraints: upper-bound-parabolic (2105)
- Range of distance target values: 2.8, 5.8 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords CARD, RIP2

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Found 1 Document (1.092 seconds)

BMRB: 25828

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RIP2 CARD, : 1 : 7 : 1 : 15 entities Detail

Interaction partners 1. RIP2 CARD, : 68 interactors Detail

Experiments performed 7 experiments Detail

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NMR combined restraints 3 contents Detail