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BMRB: 25829

2N80

p75NTR DD:RhoGDI

Authors

Lin, Z., Ibanez, C.F.

Assembly

p75NTR DD:RhoGDI

Entity

1. p75NTR DD:RhoGDI, entity 1 (polymer, Thiol state: all free), 94 monomers, 10161.24 Da Detail

GLYSSLPPAK REEVEKLLNG SAGDTWRHLA GELGYQPEHI DSFTHEACPV RALLASWATQ DSATLDALLA ALRRIQRADL VESLCSESTA TSPV

2. p75NTR DD:RhoGDI, entity 2 (polymer), 174 monomers, 19855.29 Da Detail

AQKSIQEIQE LDKDDESLRK YKEALLGRVA VSADPNVPNV VVTGLTLVCS SAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG PRAEEYEFLT PVEEAPKGML ARGSYSIKSR FTDDDKTDHL SWEWNLTIKK DWKD

Total weight

30016.53 Da

Max. entity weight

19855.29 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 52.2 %, Completeness: 40.8 %, Completeness (bb): 41.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.8 % (1266 of 3100)	44.5 % (717 of 1611)	34.7 % (422 of 1215)	46.4 % (127 of 274)
Backbone	41.8 % (662 of 1584)	50.0 % (270 of 540)	34.4 % (271 of 788)	47.3 % (121 of 256)
Sidechain	40.7 % (719 of 1768)	41.7 % (447 of 1071)	39.2 % (266 of 679)	33.3 % (6 of 18)
Aromatic	26.4 % (58 of 220)	28.2 % (31 of 110)	23.8 % (25 of 105)	40.0 % (2 of 5)
Methyl	51.0 % (155 of 304)	53.3 % (81 of 152)	48.7 % (74 of 152)

1. entity 1

GLYSSLPPAK REEVEKLLNG SAGDTWRHLA GELGYQPEHI DSFTHEACPV RALLASWATQ DSATLDALLA ALRRIQRADL VESLCSESTA TSPV

2. entity 2

AQKSIQEIQE LDKDDESLRK YKEALLGRVA VSADPNVPNV VVTGLTLVCS SAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG PRAEEYEFLT PVEEAPKGML ARGSYSIKSR FTDDDKTDHL SWEWNLTIKK DWKD

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	p75NTR DD	[U-99% 13C; U-99% 15N]	0.5 mM
2	DTT	[U-98% 2H]	1 mM
3	HEPES	[U-98% 2H]	10 mM
4	EDTA	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	RhoGDI	natural abundance	2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
7	RhoGDI	[U-99% 13C; U-99% 15N]	0.5 mM
8	p75NTR DD	natural abundance	2 mM
9	DTT	[U-98% 2H]	1 mM
10	HEPES	[U-98% 2H]	10 mM
11	EDTA	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM

Chem. Shift Complete2

Sequence coverage: 74.3 %, Completeness: 50.0 %, Completeness (bb): 51.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.0 % (3102 of 6200)	53.0 % (1708 of 3222)	44.5 % (1082 of 2430)	56.9 % (312 of 548)
Backbone	51.3 % (1625 of 3168)	59.8 % (646 of 1080)	43.1 % (680 of 1576)	58.4 % (299 of 512)
Sidechain	50.1 % (1771 of 3536)	49.6 % (1062 of 2142)	51.3 % (696 of 1358)	36.1 % (13 of 36)
Aromatic	32.5 % (143 of 440)	34.1 % (75 of 220)	30.0 % (63 of 210)	50.0 % (5 of 10)
Methyl	57.6 % (350 of 608)	58.9 % (179 of 304)	56.3 % (171 of 304)

1. entity 1

GLYSSLPPAK REEVEKLLNG SAGDTWRHLA GELGYQPEHI DSFTHEACPV RALLASWATQ DSATLDALLA ALRRIQRADL VESLCSESTA TSPV

2. entity 2

AQKSIQEIQE LDKDDESLRK YKEALLGRVA VSADPNVPNV VVTGLTLVCS SAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG PRAEEYEFLT PVEEAPKGML ARGSYSIKSR FTDDDKTDHL SWEWNLTIKK DWKD

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	p75NTR DD	[U-99% 13C; U-99% 15N]	0.5 mM
2	DTT	[U-98% 2H]	1 mM
3	HEPES	[U-98% 2H]	10 mM
4	EDTA	natural abundance	1 mM
5	sodium azide	natural abundance	1 mM
6	RhoGDI	natural abundance	2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9

#	Name	Isotope labeling	Concentration
7	RhoGDI	[U-99% 13C; U-99% 15N]	0.5 mM
8	p75NTR DD	natural abundance	2 mM
9	DTT	[U-98% 2H]	1 mM
10	HEPES	[U-98% 2H]	10 mM
11	EDTA	natural abundance	1 mM
12	sodium azide	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N80, Strand ID: A, B Detail

Release date

2015-12-20

Citation

Structural basis of death domain signaling in the p75 neurotrophin receptor
Lin, Z., Tann, J.Y., Goh, E.TH., Kelly, C., Lim, K.B., Gao, J.F., Ibanez, C.F.
Elife (2015), 4, 11692-11692, PubMed 26646181 , DOI 10.7554/eLife.11692 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 25828 released on 2015-12-20
    Title RIP2 CARD
  BMRB: 25833 released on 2015-12-20
    Title p75NTR DD:RIP2 CARD
  BMRB: 25883 released on 2015-12-20
    Title DD homodimer

Related entities 1. p75NTR DD:RhoGDI, entity 1, : 2 : 21 entities Detail

Related entities 2. p75NTR DD:RhoGDI, entity 2, : 1 : 38 entities Detail

Interaction partners 1. p75NTR DD:RhoGDI, entity 1, : 18 interactors Detail

More details for 18 interactors identified by 7 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC