BMRBj - BMREntryMaster

Found 1 Document (0.949 seconds)

BMRB: 25831

2N82

solution structure of the complex of microRNA 20b pre-element with Rbfox RRM

Authors

Yang, F., Chen, Y., Varani, G.

Assembly

complex of microRNA 20b pre-element with Rbfox RRM

Entity

1. miR-20b (polymer, Thiol state: not present), 23 monomers, 7381.307 Da Detail

GGUAGUUUUG GCAUGACUCU ACC

2. Rbfox RRM (polymer, Thiol state: not present), 104 monomers, 11811.17 Da Detail

GSHMNTENKS QPKRLHVSNI PFRFRDPDLR QMFGQFGKIL DVEIIFNERG SKGFGFVTFE NSADADRARE KLHGTVVEGR KIEVNNATAR VMTNKKTVNP YTNG

Total weight

19192.477 Da

Max. entity weight

11811.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 34.6 %, Completeness: 24.9 %, Completeness (bb): 32.7 % Detail

Polymer type: polyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	24.9 % (398 of 1600)	22.7 % (193 of 849)	30.0 % (187 of 624)	14.2 % (18 of 127)
Backbone	32.7 % (284 of 869)	37.0 % (130 of 351)	32.5 % (136 of 418)	18.0 % (18 of 100)
Sidechain	16.1 % (133 of 826)	12.9 % (64 of 498)	22.9 % (69 of 301)	0.0 % (0 of 27)
Aromatic	39.4 % (82 of 208)	40.4 % (42 of 104)	44.4 % (40 of 90)	0.0 % (0 of 14)
Methyl	17.4 % (16 of 92)	15.2 % (7 of 46)	19.6 % (9 of 46)

1. miR-20b

GGUAGUUUUG GCAUGACUCU ACC

2. Rbfox RRM

GSHMNTENKS QPKRLHVSNI PFRFRDPDLR QMFGQFGKIL DVEIIFNERG SKGFGFVTFE NSADADRARE KLHGTVVEGR KIEVNNATAR VMTNKKTVNP YTNG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	20 mM
15	Rbfox RRM	natural abundance	1 mM
16	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
18	sodium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	20 mM
20	Rbfox RRM	natural abundance	1 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 81.9 %, Completeness: 48.7 %, Completeness (bb): 51.0 % Detail

Polymer type: polyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.7 % (1557 of 3200)	47.2 % (801 of 1698)	50.5 % (630 of 1248)	49.6 % (126 of 254)
Backbone	51.0 % (887 of 1738)	48.0 % (337 of 702)	52.0 % (435 of 836)	57.5 % (115 of 200)
Sidechain	47.5 % (784 of 1652)	46.7 % (465 of 996)	51.2 % (308 of 602)	20.4 % (11 of 54)
Aromatic	38.5 % (160 of 416)	42.8 % (89 of 208)	39.4 % (71 of 180)	0.0 % (0 of 28)
Methyl	57.6 % (106 of 184)	56.5 % (52 of 92)	58.7 % (54 of 92)

1. miR-20b

GGUAGUUUUG GCAUGACUCU ACC

2. Rbfox RRM

GSHMNTENKS QPKRLHVSNI PFRFRDPDLR QMFGQFGKIL DVEIIFNERG SKGFGFVTFE NSADADRARE KLHGTVVEGR KIEVNNATAR VMTNKKTVNP YTNG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	20 mM
15	Rbfox RRM	natural abundance	1 mM
16	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
18	sodium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	20 mM
20	Rbfox RRM	natural abundance	1 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N82, Strand ID: B Detail

Release date

2016-04-11

Citation

Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer
Chen, Y., Zubovic, L., Yang, F., Godin, K., Pavelitz, T., Castellanos, J., Macchi, P., Varani, G.
Nucleic Acids Res. (2016), 44, 4381-4395, PubMed 27001519 , DOI 10.1093/nar/gkw177 , Abstract

Related entities 1. miR-20b, : 1 : 1 entities Detail

Related entities 2. Rbfox RRM, : 1 : 8 : 115 entities Detail

Interaction partners 2. Rbfox RRM, : 28 interactors Detail

More details for 28 interactors identified by 5 citations

Experiments performed 17 experiments Detail

1 2D F1-, F2-filtered 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 279 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 2D F1-, F2-filtered 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

3 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

4 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

5 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

6 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

9 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

10 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

11 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	miR-20b	natural abundance	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	20 mM
4	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

13 3D CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

14 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	20 mM
15	Rbfox RRM	natural abundance	1 mM
16	D2O	natural abundance	100 %

15 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	20 mM
15	Rbfox RRM	natural abundance	1 mM
16	D2O	natural abundance	100 %

16 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 279 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	miR-20b	[U-99% 13C; U-99% 15N]	1 mM
18	sodium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	20 mM
20	Rbfox RRM	natural abundance	1 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

17 3D 13C F1-filtered, F3-edited NOESY-HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	miR-20b	natural abundance	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	20 mM
10	Rbfox RRM	[U-99% 13C; U-99% 15N]	1 mM
11	D2O	natural abundance	100 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25831_2n82.nef
Input source #2: Coordindates	2n82.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-20-------30--------40-
GGUAGUUUUGGCAUGACUCUACC
|||||||||||||||||||||||
GGUAGUUUUGGCAUGACUCUACC
--------10--------20---

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

---110-------120-------130-------140-------150-------160-------170-------180-------190-------200----
GSHMNTENKSQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKTVNP
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSHMNTENKSQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKTVNP
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

----
YTNG
||||
YTNG
----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	23	0	0	100.0
B	B	104	0	0	100.0

Content subtype: combined_25831_2n82.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	267		100.0 (chain: A, length: 23)
2	Assigned chemical shifts	assigned_chem_shift_list_2	Warning	1160		100.0 (chain: B, length: 104)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	2363 (1)	noe	100.0 (chain: A, length: 23), 98.1 (chain: B, length: 104)
2	Distance restraints	DYANA/DIANA_distance_constraints_3	OK	62 (1)	hbond	30.4 (chain: A, length: 23), 32.7 (chain: B, length: 104)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_4	OK	149 (149)	.	82.6 (chain: A, length: 23), 56.7 (chain: B, length: 104)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 142
  - ¹³C chemical shifts: 125
- Completeness of assignments
  - Entity_assemble_ID: A
- Histogram of normalized assigned chemical shifts
Saveframe: assigned_chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 104, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 604
  - ¹³C chemical shifts: 441
  - ¹⁵N chemical shifts: 115
- Completeness of assignments
  - Entity_assemble_ID: B
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	202	142	70.3
¹³C chemical shifts	155	125	80.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	138	102	73.9
¹³C chemical shifts	115	87	75.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	40	62.5
¹³C chemical shifts	40	38	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	14	100.0
¹³C chemical shifts	14	14	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	647	604	93.4
¹³C chemical shifts	469	441	94.0
¹⁵N chemical shifts	122	115	94.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	213	207	97.2
¹³C chemical shifts	208	204	98.1
¹⁵N chemical shifts	100	97	97.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	434	397	91.5
¹³C chemical shifts	261	237	90.8
¹⁵N chemical shifts	22	18	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	49	46	93.9
¹³C chemical shifts	49	46	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	47	94.0
¹³C chemical shifts	50	29	58.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 23, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 104, Sequence coverage: 98.1%
- Total number of restraints: 2363
- Weight of restraints: 1.0 (2363)
- Potential type of restraints: upper-bound-parabolic (2363)
- Range of distance target values: 2.52, 7.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 23, Sequence coverage: 30.4%
    - Entity_assembly_ID: B, Chain length: 104, Sequence coverage: 32.7%
  - Total number of restraints: 62
  - Weight of restraints: 1.0 (62)
  - Potential type of restraints: upper-bound-parabolic (62)
  - Range of distance target values: 2.0, 3.4 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 23, Sequence coverage: 82.6%
  - Entity_assembly_ID: B, Chain length: 104, Sequence coverage: 56.7%
- Total number of restraints: 149
- Total number of restraints per polymer type: 149
- Weight of restraints: 1.0 (149)
- Potential type of restraints: square-well-parabolic (149)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords NMR, RRM, microRNA

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.949 seconds)

BMRB: 25831

Sample #1

Sample #2

Sample #3

Sample #4

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. miR-20b, : 1 : 1 entities Detail

Related entities 2. Rbfox RRM, : 1 : 8 : 115 entities Detail

Interaction partners 2. Rbfox RRM, : 28 interactors Detail

Experiments performed 17 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 6 contents Detail