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BMRB: 25833

2N83

p75NTR DD:RIP2 CARD

Authors

Lin, Z., Ibanez, C.F.

Assembly

p75NTR DD:RIP2 CARD

Entity

1. p75NTR DD:RIP2 CARD, entity 1 (polymer, Thiol state: all free), 98 monomers, 10518.60 Da Detail

KGDGGLYSSL PPAKREEVEK LLNGSAGDTW RHLAGELGYQ PEHIDSFTHE ACPVRALLAS WATQDSATLD ALLAALRRIQ RADLVESLCS ESTATSPV

2. p75NTR DD:RIP2 CARD, entity 2 (polymer, Thiol state: all free), 106 monomers, 12102.76 Da Detail

GIAQQWIQSK REDIVNQMTE ACLNQSLDAL LSRDLIMKED YELVSTKPTR TSKVRQLLDT TDIQGEEFAK VIVQKLKDNK QMGLQPYPEI LVVSRSPSLN LLQNKS

Total weight

22621.36 Da

Max. entity weight

12102.76 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 59.3 %, Completeness: 50.2 %, Completeness (bb): 51.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.2 % (1178 of 2345)	51.9 % (634 of 1222)	46.4 % (420 of 905)	56.9 % (124 of 218)
Backbone	51.3 % (619 of 1206)	57.0 % (233 of 409)	44.9 % (270 of 602)	59.5 % (116 of 195)
Sidechain	50.4 % (672 of 1333)	49.3 % (401 of 813)	52.9 % (263 of 497)	34.8 % (8 of 23)
Aromatic	44.0 % (44 of 100)	44.0 % (22 of 50)	42.6 % (20 of 47)	66.7 % (2 of 3)
Methyl	58.4 % (146 of 250)	58.4 % (73 of 125)	58.4 % (73 of 125)

1. entity 1

KGDGGLYSSL PPAKREEVEK LLNGSAGDTW RHLAGELGYQ PEHIDSFTHE ACPVRALLAS WATQDSATLD ALLAALRRIQ RADLVESLCS ESTATSPV

2. entity 2

GIAQQWIQSK REDIVNQMTE ACLNQSLDAL LSRDLIMKED YELVSTKPTR TSKVRQLLDT TDIQGEEFAK VIVQKLKDNK QMGLQPYPEI LVVSRSPSLN LLQNKS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K

#	Name	Isotope labeling	Concentration
1	p75NTR DD	[U-99% 13C; U-99% 15N]	0.5 mM
2	DTT	[U-98% 2H]	10 mM
3	RIP2 CARD	natural abundance	1 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K

#	Name	Isotope labeling	Concentration
4	RIP2 CARD	[U-99% 13C; U-99% 15N]	0.5 mM
5	p75NTR DD	natural abundance	1 mM
6	DTT	[U-98% 2H]	10 mM

Chem. Shift Complete2

Sequence coverage: 60.3 %, Completeness: 52.8 %, Completeness (bb): 51.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.8 % (2477 of 4690)	55.7 % (1362 of 2444)	47.2 % (855 of 1810)	59.6 % (260 of 436)
Backbone	51.7 % (1246 of 2412)	57.9 % (474 of 818)	44.6 % (537 of 1204)	60.3 % (235 of 390)
Sidechain	54.7 % (1459 of 2666)	54.6 % (888 of 1626)	54.9 % (546 of 994)	54.3 % (25 of 46)
Aromatic	31.0 % (62 of 200)	34.0 % (34 of 100)	26.6 % (25 of 94)	50.0 % (3 of 6)
Methyl	58.4 % (292 of 500)	58.4 % (146 of 250)	58.4 % (146 of 250)

1. entity 1

KGDGGLYSSL PPAKREEVEK LLNGSAGDTW RHLAGELGYQ PEHIDSFTHE ACPVRALLAS WATQDSATLD ALLAALRRIQ RADLVESLCS ESTATSPV

2. entity 2

GIAQQWIQSK REDIVNQMTE ACLNQSLDAL LSRDLIMKED YELVSTKPTR TSKVRQLLDT TDIQGEEFAK VIVQKLKDNK QMGLQPYPEI LVVSRSPSLN LLQNKS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K

#	Name	Isotope labeling	Concentration
1	p75NTR DD	[U-99% 13C; U-99% 15N]	0.5 mM
2	DTT	[U-98% 2H]	10 mM
3	RIP2 CARD	natural abundance	1 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K

#	Name	Isotope labeling	Concentration
4	RIP2 CARD	[U-99% 13C; U-99% 15N]	0.5 mM
5	p75NTR DD	natural abundance	1 mM
6	DTT	[U-98% 2H]	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.66 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.66 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N83, Strand ID: A, B Detail

Release date

2015-12-20

Citation

Structural basis of death domain signaling in the p75 neurotrophin receptor
Lin, Z., Tann, J.Y., Goh, E.TH., Kelly, C., Lim, K.B., Gao, J.F., Ibanez, C.F.
Elife (2015), 4, 11692-11692, PubMed 26646181 , DOI 10.7554/eLife.11692 , Abstract

Related entities 1. p75NTR DD:RIP2 CARD, entity 1, : 2 : 19 entities Detail

Related entities 2. p75NTR DD:RIP2 CARD, entity 2, : 3 : 7 entities Detail

Interaction partners 1. p75NTR DD:RIP2 CARD, entity 1, : 18 interactors Detail

More details for 18 interactors identified by 7 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC