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Tetrameric i-motif structure of dT-dC-dC-CFL-CFL-dC at acidic pH
Authors
Abou-Assi, R., Harkness, R.W., Martin-Pintado, N.W., Wilds, C.J., Campos-Olivas, R., Mittermaier, A.K., Gonzalez, C.W., Damha, M.J.
Assembly
Tetrameric i-motif structure of dT-dC-dC-CFL-CFL-dC
Entity
1. Tetrameric i-motif structure of dT-dC-dC-CFL-CFL-dC (polymer, Thiol state: not present), 6 monomers, 1804.099 × 4 Da Detail

TCCXXC


Total weight
7216.396 Da
Max. entity weight
1804.099 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 66.7 %, Completeness: 79.1 %, Completeness (bb): 67.9 % Detail

Polymer type: polydeoxyribonucleotide

Total1H
All79.1 % (34 of 43)79.1 % (34 of 43)
Suger, PO467.9 % (19 of 28)67.9 % (19 of 28)
Nucleobase100.0 % (15 of 15)100.0 % (15 of 15)
Aromatic100.0 % (8 of 8)100.0 % (8 of 8)
Methyl100.0 % (1 of 1)100.0 % (1 of 1)

1. DNA (5'-D(*TP*CP*CP*(CFL)P*(CFL)P*C)-3')

TCCXXC

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5.0


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*TP*CP*CP*(CFL)P*(CFL)P*C)-3')natural abundance1.25 mM
2sodium chloridenatural abundance10 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 278 K, pH 5.0


#NameIsotope labelingTypeConcentration
3DNA (5'-D(*TP*CP*CP*(CFL)P*(CFL)P*C)-3')natural abundance1.25 mM
4sodium chloridenatural abundance10 mM

Release date
2016-08-14
Citation
Stabilization of i-motif structures by 2'-β-fluorination of DNA
Abou-Assi, H., Harkness, R.W., Martin-Pintado, N., Wilds, C.J., Campos-Olivas, R., Mittermaier, A.K., Gonzalez, C., Damha, M.J.
Nucleic Acids Res. (2016), 44, 4998-5009, PubMed 27166371 , DOI 10.1093/nar/gkw402 ,
Experiments performed 5 experiments Detail
NMR combined restraints 3 contents Detail
Keywords 2'F-ANA, 2'F-araC, Modified nucleotides, i-motif