BMRB: 25857

NMR Solution Structure of Lacticin Q and Aureocin A53

Authors

Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.

Assembly

Aureocin A53

Entity

1. Aureocin A53 (polymer, Thiol state: not present), 51 monomers, 5984.146 Da Detail

MSWLNFLKYI AKYGKKAVSA AWKYKGKVLE WLNVGPTLEW VWQKLKKIAG L

Formula weight

5984.146 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.2 %, Completeness (bb): 96.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	95.2 % (334 of 351)	95.2 % (334 of 351)
Backbone	96.2 % (101 of 105)	96.2 % (101 of 105)
Sidechain	94.7 % (233 of 246)	94.7 % (233 of 246)
Aromatic	87.2 % (41 of 47)	87.2 % (41 of 47)
Methyl	100.0 % (32 of 32)	100.0 % (32 of 32)

1. Aureocin A53

MSWLNFLKYI AKYGKKAVSA AWKYKGKVLE WLNVGPTLEW VWQKLKKIAG L

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Aureocin A53	natural abundance		1 mM
2	DSS	natural abundance		0.01 % w/v
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2N8O, Strand ID: A Detail

---

Release date

2016-02-14

Citation

Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity
Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.
Biochemistry (2016), 55, 733-742, PubMed 26771761 , DOI 10.1021/acs.biochem.5b01306 , Abstract

Related entities 1. Aureocin A53, : 1 : 5 : 5 entities Detail

Experiments performed 3 experiments Detail